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Method used for evaluating immunomodulatory effect of lactobacillus acidophilus and application of method

A Lactobacillus acidophilus, immunomodulatory technology, applied in biochemical equipment and methods, microbial determination/inspection, instruments, etc., can solve problems such as limitations, and achieve the effect of saving time

Inactive Publication Date: 2017-03-08
黑龙江省绿色食品科学研究院
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Problems solved by technology

[0004] At present, the evaluation of the immunomodulatory effect of Lactobacillus acidophilus is mainly based on the organ / body weight ratio, cellular immune function test, humoral immune function test, and macrophage function test stipulated in the "Health Food Functional Evaluation Procedures and Test Methods" It is a method related to the determination of NK cell activity, but only based on the positive or negative results of a certain detection index to judge whether it has an immunomodulatory effect has certain limitations for comprehensive, effective and holistic evaluation

Method used

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  • Method used for evaluating immunomodulatory effect of lactobacillus acidophilus and application of method
  • Method used for evaluating immunomodulatory effect of lactobacillus acidophilus and application of method
  • Method used for evaluating immunomodulatory effect of lactobacillus acidophilus and application of method

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Embodiment Construction

[0036] The present invention will be further described below in conjunction with specific examples, but the present invention is not limited by the examples.

[0037] 1 Experimental materials and methods

[0038] 1.1 Experimental strains, cells, experimental animals, gene chips

[0039] Lactobacillus acidophilus NCFM was donated by Danisco (Danisco).

[0040] Human colon cancer cells Caco-2 were purchased from Shanghai Institute of Biological Sciences, Chinese Academy of Sciences.

[0041] Kunming mice were purchased from the Experimental Animal Center of Heilongjiang Medical University.

[0042] Gene chip: Affymetrix's U133Plus2.0 gene expression profile chip, provided by Shanghai Biochip Co., Ltd. - Biochip Shanghai National Engineering Research Center, the chip covers 47,000 transcripts, representing 38,500 human genes.

[0043] 1.2 Culture of human colon adenocarcinoma cells Caco-2

[0044] Human colon cancer cell Caco-2 is an adherent growth cell, cultured in a 5% CO2 i...

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Abstract

The invention discloses a method used for evaluating an immunomodulatory effect of lactobacillus acidophilus and an application of the method, and belongs to the field of biotechnology. According to the method provided by the invention, a dynamic change situation of immunity-related differentially expressed genes is detected, wherein the differentially expressed genes include up-regulated immunity-related genes CCL2, CCL20, IL1 alpha, IL1 beta, IL6ST, IL8, PTX3 and TNFRSF9 and a down-regulated immunity-related gene CXCR4. Through the method, the immunomodulatory effect of the lactobacillus acidophilus can be evaluated only by detecting nine immunity-related genes, and quick detection can be realized by utilizing Real Time PCR, so that the time is greatly shortened and the deficiency of existing evaluation of the immunomodulatory effect of the lactobacillus acidophilus is overcome; and the method is well verified in a mouse test and a Caco-2 cell.

Description

technical field [0001] The invention relates to a method and application for evaluating the immune regulation effect of Lactobacillus acidophilus, belonging to the field of biotechnology. Background technique [0002] Probiotic lactic acid bacteria are a group of living microorganisms that can adhere to the host intestine and change the flora composition of a certain part of the host through colonization, thereby exerting various beneficial effects on the host. Studies have shown that it plays an important role in maintaining the balance of intestinal microecology, enhancing the body's immune function, lowering serum cholesterol, and preventing and inhibiting tumor occurrence. It is one of the essential physiological flora to maintain the health of the body. Disturbance of normal intestinal flora can lead to loss of oral immune tolerance, and even cause systemic hypersensitivity. These bacteria are an indispensable and important part of the maintenance of gastrointestinal or...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/02
CPCC12Q1/6888C12Q1/686C12Q2600/166G01N33/5005C12Q2561/113C12Q2521/107C12Q2531/113
Inventor 姜毓君满朝新王辉林官根李明雨
Owner 黑龙江省绿色食品科学研究院
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