Method used for evaluating immunomodulatory effect of lactobacillus acidophilus and application of method

A Lactobacillus acidophilus, immunomodulatory technology, applied in biochemical equipment and methods, microbial determination/inspection, instruments, etc., can solve problems such as limitations, and achieve the effect of saving time
CN106480190AInactive Publication Date: 2017-03-08黑龙江省绿色食品科学研究院
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
黑龙江省绿色食品科学研究院
Publication Date
2017-03-08
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method used for evaluating an immunomodulatory effect of lactobacillus acidophilus and an application of the method, and belongs to the field of biotechnology. According to the method provided by the invention, a dynamic change situation of immunity-related differentially expressed genes is detected, wherein the differentially expressed genes include up-regulated immunity-related genes CCL2, CCL20, IL1 alpha, IL1 beta, IL6ST, IL8, PTX3 and TNFRSF9 and a down-regulated immunity-related gene CXCR4. Through the method, the immunomodulatory effect of the lactobacillus acidophilus can be evaluated only by detecting nine immunity-related genes, and quick detection can be realized by utilizing Real Time PCR, so that the time is greatly shortened and the deficiency of existing evaluation of the immunomodulatory effect of the lactobacillus acidophilus is overcome; and the method is well verified in a mouse test and a Caco-2 cell.
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Description

technical field

[0001] The invention relates to a method and application for evaluating the immune regulation effect of Lactobacillus acidophilus, belonging to the field of biotechnology. Background technique

[0002] Probiotic lactic acid bacteria are a group of living microorganisms that can adhere to the host intestine and change the flora composition of a certain part of the host through colonization, thereby exerting various beneficial effects on the host. Studies have shown that it plays an important role in maintaining the balance of intestinal microecology, enhancing the body's immune function, lowering serum cholesterol, and preventing and inhibiting tumor occurrence. It is one of the essential physiological flora to maintain the health of the body. Disturbance of normal intestinal flora can lead to loss of oral immune tolerance, and even cause systemic hypersensitivity. These bacteria are an indispensable and important part of the maintenance of gastrointestinal or...

Claims

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