Building method and application of seminal plasma metabonomics-based OPLS-DA diagnosis model

A diagnostic model, metabolomics technology, applied in the field of establishment of OPLS-DA diagnostic model

Inactive Publication Date: 2017-03-08
NANJING MEDICAL UNIV
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Problems solved by technology

It is unclear whether this reclassification has a better effect on the evaluation of infertile couples in this case, and the role of definitive diagnosis and male factor evaluation in infertile couples

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  • Building method and application of seminal plasma metabonomics-based OPLS-DA diagnosis model

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Embodiment Construction

[0026] In order to make the technical means, creative features, goals and effects achieved by the present invention easy to understand, the present invention will be further described below in conjunction with specific embodiments.

[0027]The embodiment of the present invention complies with government regulations and has been approved by the Ethics Society of Nanjing Medical University. The male case came from a couple who were unable to conceive for more than 12 months from the outpatient clinic of the Affiliated Hospital of Nanjing Medical University, and the female spouse underwent a series of clinical examinations and abnormal reproductive status was excluded. The male case underwent a complete medical history and physical examination, hormone determination and semen routine analysis; the spouse underwent a complete medical history and physical examination, including routine gynecological examination, measurement of serum hormone levels, test of tubal ovarian function, be...

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Abstract

The invention discloses a building method and an application of a seminal plasma metabonomics-based OPLS-DA diagnosis model. The method comprises the following steps of modeling object screening and sample collection; quality control sample preparation; sample extraction; sample derivatization; gas chromatography-mass spectrometry analysis; use of the quality control sample; compound identification; and OPLS-DA modeling. According to the seminal plasma metabonomics-based OPLS-DA diagnosis model, the judgment effect of the seminal plasma metabonomics-based OPLS-DA diagnosis model on UMI is superior to that of an OPLS-DA model built by using parameters such as a seminal fluid quantity, sperm density, a sperm motility rate, sperm activity, a sperm movement mode and the like in seminal fluid parameters; the sensitivity and specificity of the seminal plasma metabonomics-based OPLS-DA diagnosis model are 98% and 95% respectively; and the sensitivity and specificity of the OPLS-DA model are only 67% and 73%.

Description

technical field [0001] The invention relates to the field of male infertility biotechnology, in particular to a method for establishing an OPLS-DA diagnostic model based on seminal plasma metabolomics and its application. Background technique [0002] Unexplained male infertility (Unexplained Male Infertility, UMI) refers to the phenomenon of male infertility after routine semen analysis results are within the normal range and physical and endocrine abnormalities are excluded. In addition to erectile problems and intercourse factors, immune causes and sperm dysfunction may also contribute to this condition. The causes of unexplained male infertility include low-grade leukospermia and mitochondrial DNA polymerase gene polymorphisms, among others. Infertility is a prevalent problem among couples worldwide, clinically defined as the inability of a couple to conceive after having regular intercourse for a year, it is estimated that 4-17% of couples visit a doctor to correct the...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06F19/00
CPCG16H50/50G16C20/40
Inventor 乔善磊夏彦恺王心如陈敏健
Owner NANJING MEDICAL UNIV
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