Application of a kind of rice active peptide in the preparation of antioxidant preparation for protecting endothelial progenitor cells
An endothelial progenitor cell and active peptide technology, which is applied to the application field of rice active peptide in the preparation of antioxidant preparations for protecting endothelial progenitor cells, can solve the problem that the antioxidative damage activity of rice active peptide is in a blank stage, and achieves protection of endothelial progenitor cells. effect of cells
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[0006] Rice active peptide (SEQ ID NO.1) to H 2 o 2 Antioxidative protection of induced endothelial progenitor cells
[0007] 1. Select the cells in the logarithmic growth phase, digest them with EDTA-free trypsin to make a cell suspension, measure the cell concentration in the suspension with a counting plate, inoculate 5000 cells in a 96-well plate, and inoculate 200 μL in each well, Set up 6 replicate wells in each group, at 37°C 5% CO 2 After culturing in the incubator for 4 hours, they were divided into normal group, hydrogen peroxide treatment group, hydrogen peroxide + rice active peptide protection group, the original medium was sucked out, and 160 μL of medium was first added to all groups, and the normal group, hydrogen peroxide The treatment group was added with 20 μL of serum-free medium, and the hydrogen peroxide + rice active peptide protection group was added with corresponding concentration and equal volume of rice active peptide, so that the final concentrat...
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