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Application of rice bioactive peptide in preparing antioxidant for protecting endothelial progenitor cells

A technology of endothelial progenitor cells and active peptides, which is applied in the application field of rice active peptides in the preparation of antioxidant preparations for protecting endothelial progenitor cells. cell effect

Active Publication Date: 2017-03-15
长沙飞创生物技术有限责任公司
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  • Abstract
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Problems solved by technology

[0004] After investigation, there is no relevant literature report by Chinese scholars on the protection of endothelial progenitor cells from oxidative stress damage by rice active peptides. Therefore, the research field on the activity of rice active peptides against oxidative damage is still in a blank stage.

Method used

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  • Application of rice bioactive peptide in preparing antioxidant for protecting endothelial progenitor cells
  • Application of rice bioactive peptide in preparing antioxidant for protecting endothelial progenitor cells
  • Application of rice bioactive peptide in preparing antioxidant for protecting endothelial progenitor cells

Examples

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Embodiment Construction

[0006] Rice active peptide (SEQ ID NO.1) to H 2 o 2 Antioxidative protection of induced endothelial progenitor cells

[0007] 1. Select the cells in the logarithmic growth phase, digest them with EDTA-free trypsin to make a cell suspension, measure the cell concentration in the suspension with a counting plate, inoculate 5000 cells in a 96-well plate, and inoculate 200 μL in each well, Set up 6 replicate wells in each group, at 37°C 5% CO 2 After culturing in the incubator for 4 hours, they were divided into normal group, hydrogen peroxide treatment group, hydrogen peroxide + rice active peptide protection group, the original medium was sucked out, and 160 μL of medium was first added to all groups, and the normal group, hydrogen peroxide The treatment group was added with 20 μL of serum-free medium, and the hydrogen peroxide + rice active peptide protection group was added with corresponding concentration and equal volume of rice active peptide, so that the final concentrat...

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Abstract

The invention discloses an application of rice bioactive peptide in preparing an antioxidant for protecting endothelial progenitor cells. According to the application of the rice bioactive peptide in preparing the antioxidant for protecting the endothelial progenitor cells, the rice bioactive peptide has the function of protecting the endothelial progenitor cells against oxidization, and a novel food-borne application is provided for protecting the endothelial progenitor cells against related diseases.

Description

technical field [0001] The application of the rice active peptide in the preparation of anti-oxidation preparation or food for protecting endothelial progenitor cells. Background technique [0002] Rice protein has excellent nutritional value, high absorption and utilization rate by human body, high biological potency, and rice protein is rich in various amino acids. However, rice protein itself has poor solubility, which seriously affects its utilization. Therefore, it is of great significance to further process rice to further enhance its value. The hydrolysis of rice protein and the extraction of biologically active peptides can effectively realize the deep processing of rice protein. Rice Active Peptide is a biologically active peptide extracted from rice. Rice active peptides include several active peptides with different functions, including rice antioxidant active peptides, rice antihypertensive active peptides, rice immune active peptides, opioid active peptides, o...

Claims

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Application Information

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IPC IPC(8): A23L33/18
Inventor 梁盈李佳佳林亲录黄萍吴伟王玉倩
Owner 长沙飞创生物技术有限责任公司
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