A kind of anther separation method suitable for stevia anther culture
A separation method and technology of stevia flowers, applied in the field of plant tissue culture, can solve the problems of difficulty in directly obtaining inbred lines and homozygous plants, separation of group traits of hybrid offspring, slowing down the breeding process of stevia, etc., and achieve a simple and efficient separation method, reduce Difficulty of operation and effect of ensuring integrity
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[0022] The present invention is a method for separating anthers suitable for stevia anther culture, comprising the following steps:
[0023] (1) Preparation of sterile petri dish: spread 3-4 pieces of filter paper in a clean petri dish, wrap it with newspaper, and sterilize it according to the conventional high temperature and high pressure sterilization method for future use.
[0024] (2) Inflorescence collection: Yu Stevia (variety 'Zhongshan No. 5', see Breeding of a new variety of Stevia glycoside Zhongshan No. 5 - "China Sugar" 2015 Issue 04) During the full flowering stage, the bracts are tightly closed and the flower buds are about 2 mm long. In a dish, store in a 4°C refrigerator for later use.
[0025] (3) Disinfection of inflorescences: In an ultra-clean workbench, first sterilize the inflorescences with 75% ethanol for 30 seconds, soak them in 1% mercuric chloride for 3-4 minutes, then wash them with sterile water for 3-4 times, and then sterilize the inflorescenc...
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