A method for determining the content of norepinephrine bitartrate by b-z chemical oscillation reaction
A norepinephrine and bitartrate technology, applied in the direction of material electrochemical variables, etc., can solve the problems of cumbersome detection operations and high costs
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Embodiment 1B
[0017] Example 1 B-Z Chemical Oscillation Reaction Determination of Experimental Device and Experimental Conditions for the Content of Norepinephrine Bitartrate
[0018] Carried out in a glass reactor (volume about 20mL) with a constant temperature jacket and magnetic stirring, adding a certain concentration of HOOCCH to the reactor in sequence 2 COOH solution, KBrO 3 solution, H 2 SO 4 Each solution was 5mL, and at the same time, 5mL (NH 4 ) 2 Ce(SO 4 ) 3 Put it in a constant temperature tank for constant temperature. After 10 minutes, click the "Start Communication" button on the computer interface to set the communication temperature. After clicking the "OK" button, the baseline will appear on the computer interface. A good ammonium cerium sulfate solution was used to keep the total volume of the reaction bottom liquid at 20 mL. Maintain the system temperature, use the platinum electrode as the working electrode and the 217-type calomel electrode as the reference ele...
Embodiment 2
[0020] The standard curve of embodiment 2 oscillation cycle variation and norepinephrine bitartrate concentration
[0021] Under the optimal experimental conditions, take the negative logarithm of the concentration of norepinephrine bitartrate as the abscissa, and the change amount ΔT of the oscillation period as the ordinate to examine the linear relationship. For specific results, see figure 2 .
[0022] Depend on figure 2 It can be seen that the concentration of norepinephrine bitartrate is 3.92×10 -5 ~3.33×10 -3 In the range of mol / L, it has a good linear relationship with the change amount ΔT of the oscillation period, and the linear regression equation is ΔT=-59.709(-lgC)+253.67(R 2 =0.9823, n=6) its detection limit is 4.76×10 -10 mol / L.
Embodiment 3B
[0023] Example 3 B-Z Chemical Oscillation Reaction Determination of Norepinephrine Bitartrate Content and Recovery Rate Experiment in Serum Samples
[0024] Serum samples for testing were provided free of charge by a hospital in Liaocheng. After high-speed centrifugation to remove macromolecules such as proteins (14000r / min), a certain volume of supernatant was accurately measured in a 50mL volumetric flask, and diluted with PBS buffer solution of pH=7 to scale, constant volume to obtain the sample solution.
[0025] Add low, medium and high levels of standard norepinephrine bitartrate to the sample solution respectively, and use the linearity between the negative logarithm of the concentration of norepinephrine bitartrate and the change of the oscillation period in the B-Z chemical oscillation reaction system The relationship between the determination of sample content and recovery rate, the results are shown in Table 1. The average recoveries were 98.04%, 97.56% and 97.87%,...
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