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High-transdermal-absorption subcutaneouse targeted release factor, construction method and application

A construction method and high-permeability technology, applied in the design and cosmetic application of hEGF, can solve problems such as poor transdermal absorption, and achieve the effects of promoting proliferation, promoting cell proliferation, improving transmembrane transport and transdermal absorption.

Inactive Publication Date: 2017-04-05
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Application Information

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Problems solved by technology

However, as a biomacromolecule, poor transdermal absorption has always been a major problem plaguing its practical application.

Method used

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  • High-transdermal-absorption subcutaneouse targeted release factor, construction method and application
  • High-transdermal-absorption subcutaneouse targeted release factor, construction method and application
  • High-transdermal-absorption subcutaneouse targeted release factor, construction method and application

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Embodiment Construction

[0030] The present invention will be further described in detail below through the specific examples, the following examples are only descriptive, not restrictive, and cannot limit the protection scope of the present invention with this.

[0031] The present invention designs a recombinant protein with high transdermal absorption and subcutaneous targeted release, and establishes its genetic engineering expression method. The specific contents include:

[0032] (1) Cell-penetrating peptides were introduced into the N-terminus of the hEGF gene by molecular biology methods, and the subcutaneous endogenously expressed protease cleavage site was used as the connecting arm of the two, and the three were spliced ​​together.

[0033] The cell-penetrating peptide is a class of peptide substances that can carry a variety of biologically active substances (proteins, nucleic acids, polysaccharides, etc.) of different sizes and properties into cells, including (but not limited to) Pep-1, T...

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Abstract

The invention relates to a high-transdermal-absorption subcutaneouse targeted release factor, a construction method and application. According to the codon preference of an expression host, gene segments of three parts including hEGF, cell permeable peptides and a subcutaneouse endogenous protease cleavage site are optimized, spliced together and connected in expression plasmid to construct an expression carrier of high-transdermal-absorption subcutaneouse targeted release hEGF, and the expression carrier is converted in hosts such as escherichia coli so that efficient expression production can be achieved. By means of the method, the defect that the percutaneous permeability of hEGF used as a biomacromolecule substance is low is overcome, the hEGF can efficiently span a skin cuticula and can be released under skin in a targeted manner to promote fibroblast proliferation and other effects, and therefore the actual effects on the aspects of skin protection, acne removing, wrinkle removing, skin whitening and the like are remarkably improved, and a new perspective and a new method are provided for development and production of related beauty products and biological medicine.

Description

technical field [0001] The invention belongs to the fields of biomedicine and cosmetics, in particular to the design and cosmetic application of highly efficient transdermal absorption of hEGF for subcutaneous targeted release. Background technique [0002] Epidermal growth factor (EGF) is a single-chain polypeptide containing 53 amino acids. It not only acts on epidermal cells, but also acts on skin fiber cells, keratinocytes, etc., and automatically interacts with the EGF receptor (EGF- R) combined with a strong ability to promote cell proliferation, so it can be used as a cosmetic additive and drug to perform skin repair, recuperation, acne removal (removal of acne, sores and pimples), whitening and other effects. However, as a biomacromolecule, poor transdermal absorption has always been a major problem plaguing its practical application. Cell-penetrating peptides (CPP) are small molecular polypeptides that can carry various exogenous substances such as proteins into ce...

Claims

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Application Information

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IPC IPC(8): C07K19/00C12N15/63A61K8/64A61K38/18A61K47/64A61Q19/02A61Q19/08A61P17/10
Inventor 罗学刚王玥闫莉华张同存
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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