Preparation method and application of papaya seed benzyl glucosinolate extract

A papaya seed and glucoside technology, applied in the field of preparation and application of papaya seed benzyl glucosinolate extract, can solve the problem of undiscovered immune activity

Inactive Publication Date: 2017-04-26
HAINAN MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

No reports of immune ac...

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  • Preparation method and application of papaya seed benzyl glucosinolate extract
  • Preparation method and application of papaya seed benzyl glucosinolate extract
  • Preparation method and application of papaya seed benzyl glucosinolate extract

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Embodiment 1

[0014] Embodiment 1: the preparation of papaya seed benzyl glucosinolate extract: take by weighing 1 kg of pulverized papaya seeds, add 75% ethanol with a volume ratio of 9 times the weight of papaya seeds, extract twice, and reduce Concentrated under reduced pressure, the concentrated solution was extracted three times with petroleum ether, ethyl acetate, and n-butanol successively, and finally the water layer was concentrated under reduced pressure at 50°C to 300mL, and 75% ethanol with a volume ratio of twice the volume was added, and suction filtered. The filtrate was concentrated at 50°C until there was no alcohol smell. The upper MCI column (5cm×45cm) was eluted with ultrapure water at a flow rate of 8mL / min, and the eluate was collected at 200mL / bottle. Detected by HPLC, combined according to the chromatogram results, concentrated under reduced pressure at 50°C, and freeze-dried at -45°C to a light yellow powder to obtain papaya seed benzyl glucosinolate extract, which ...

Embodiment 2

[0015] Embodiment 2: the preparation of papaya seed benzyl glucosinolate extract: take by weighing 1 kg of pulverized papaya seeds, add 65% ethanol with a volume ratio of 9 times the weight of papaya seeds, extract twice, and reduce Concentrated under reduced pressure, the concentrated solution was extracted three times with petroleum ether, ethyl acetate, and n-butanol in sequence, and finally the water layer was concentrated under reduced pressure at 50°C to 300mL, and 85% ethanol with a volume ratio of 1 times the volume was added, and suction filtered. The filtrate was concentrated at 50°C until there was no alcohol smell. The upper MCI column (5cm×45cm) was eluted with ultrapure water at a flow rate of 8mL / min, and the eluate was collected at 200mL / bottle. Detected by HPLC, combined according to the chromatogram results, concentrated under reduced pressure at 50°C, and freeze-dried at -45°C to a light yellow powder to obtain papaya seed benzyl glucosinolate extract, which...

Embodiment 3

[0016] Embodiment 3: the preparation of papaya seed benzyl glucosinolate extract: take by weighing 1 kg of pulverized papaya seeds, add 85% ethanol with a volume ratio of 9 times the weight of papaya seeds, extract twice, and reduce Concentrated under reduced pressure, the concentrated solution was extracted 3 times with petroleum ether, ethyl acetate, and n-butanol in sequence, and finally the water layer was concentrated under reduced pressure at 50°C to 300mL, and 65% ethanol with a volume ratio of 3 times was added, and suction filtered. The filtrate was concentrated at 50°C until there was no alcohol smell. The upper MCI column (5cm×45cm) was eluted with ultrapure water at a flow rate of 8mL / min, and the eluate was collected at 200mL / bottle. Detected by HPLC, combined according to the chromatogram results, concentrated under reduced pressure at 50°C, and freeze-dried at -45°C to a light yellow powder to obtain papaya seed benzyl glucosinolate extract, which was analyzed b...

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Abstract

The invention discloses a preparation method of papaya seed benzyl glucosinolate extract and its function of enhancing body immunity. With papaya seed benzyl glucosinolate extract as an object, the influence of the papaya seed benzyl glucosinolate extract upon mice humoral immunity, nonspecific immunity and immune organ index is studied according to the pharmacological methods provided in Technical Standards for Testing and Assessment of Immune-Health Foods by the Ministry of Health of the PRC. The results indicate that the papaya seed benzyl glucosinolate extract can significantly increase thymus index in mice, promote the generation of serum hemolysin and enhance the phagocytosis of peritoneal macrophage. It is indicated that the papaya seed benzyl glucosinolate extract can enhance body immunity.

Description

technical field [0001] The invention relates to a new application of papaya, in particular to a preparation method of papaya seed benzyl glucosinolate extract and a new application for enhancing immunity. Background technique [0002] Papaya (Carica papaya L), also known as Longevity Fruit, is the fruit of the Papaya family plant and is widely planted in Guangdong, Guangxi, Hainan, Yunnan, Fujian and other provinces in my country. Studies have shown that papaya fruit contains papain, papain, rennet, carotene, etc., and also contains more than 17 kinds of amino acids and various nutrients, which have the effects of protecting the liver, anti-inflammatory and antibacterial, and lowering blood lipids. People eat papaya pulp, and the seeds are usually waste. Papaya is rich in benzyl glucosinolate (benzyl glucosinolate, benzyl glucosinolate), especially the highest content in papaya seeds. Under the action of glucosidase or intestinal bacteria, it is hydrolyzed into isothiocyana...

Claims

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Application Information

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IPC IPC(8): A61K31/7028A61P37/04A23L33/105
CPCA61K31/7028A23V2002/00A61K36/185A23V2250/21A23V2200/324
Inventor 李泽友赵珂何梦雪
Owner HAINAN MEDICAL COLLEGE
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