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Method for constructing photosynthetic pathway gene regulation network

A method for constructing and regulating network technology, which is applied in the field of constructing gene regulation network of photosynthetic pathway, can solve the problems of deviation and error of gene regulation network, and achieve the effect of constructing network accurately and credibly.

Active Publication Date: 2017-04-26
BEIJING FORESTRY UNIVERSITY
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Problems solved by technology

[0003] At present, the construction of gene regulatory network mainly consists of methods such as Boolean network, differential equation and Bayesian network. There is a certain degree of deviation, and the variation of the gene itself is not involved in the construction of the gene regulatory network

Method used

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  • Method for constructing photosynthetic pathway gene regulation network
  • Method for constructing photosynthetic pathway gene regulation network
  • Method for constructing photosynthetic pathway gene regulation network

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Embodiment 1

[0070] Select 435 individuals of Populus tomentosa, extract genomic DNA, and use it for resequencing, and obtain genomic SNP data through sequence comparison. The photosynthetic pathway genes of Populus tomentosa, a closely related species of Populus tomentosa, were found from the KEGG Pathway (see Table 1 for the selected genes), and the corresponding genes of Populus tomentosa were located by multiple alignment methods, and the SNP data were extracted. Further, the SNP data with genotype frequency less than 5% were deleted.

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Abstract

The invention provides a method for constructing a photosynthetic pathway gene regulation network. The method comprises the following steps of 1) obtaining SNP data of photosynthetic pathway genes of species; 2) measuring phenotypic traits of the species to obtain phenotypic data; 3) performing inter-genetic locus epistasis mode detection by utilizing the SNP data obtained in the step 1) and the phenotypic data obtained in the step 2), and determining an interaction relationship between every two genes; 4) verifying the correlation among the genes obtained in the step 3); and 5) constructing the gene regulation network by taking the correlative genes obtained in the step 3) as candidate genes, wherein the step 1) and the step 2) are free from time sequence limitation. According to the construction method provided by the invention, genetic locus mutation and biotic population phenotypes are comprehensively considered; the gene regulation network is constructed more accurately by calculating inter-locus epistasis; and the method has important theoretical significances and practical values for research and improvement of a photosynthesis mechanism.

Description

technical field [0001] The invention relates to the technical field of gene regulation network, in particular to a method for constructing a photosynthetic pathway gene regulation network. Background technique [0002] Photosynthesis is one of the most important chemical reactions in the world. More than 90% of the biomass energy and wood tissue on the earth come from photosynthesis, but the efficiency of photosynthesis is low, and only 2% to 4% of solar energy can be used. Therefore, we are committed to It is particularly important for the improvement of photosynthesis. However, photosynthesis involves a series of complex biological processes, and its regulatory relationship is complicated. Only by improving the photosynthetic rate through traditional methods, its effect is limited. Photosynthesis is a complex process that is regulated by multiple levels and multiple genes, and it cannot be regulated by the study of single genes alone. Gene regulatory network embodies the...

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Application Information

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IPC IPC(8): G06F19/12
CPCG16B5/00
Inventor 张德强王龙欣杜庆章
Owner BEIJING FORESTRY UNIVERSITY
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