Bacteriostatic stevia rebaudiana culture medium

Abstract

The invention belongs to the technical field of a stevia rebaudiana culture medium and provides a bacteriostatic stevia rebaudiana culture medium. The bacteriostatic stevia rebaudiana culture medium comprises a base component and a Chinese herbal medicine extracting solution, wherein the Chinese herbal medicine extracting solution comprises the following components in parts by weight: 11-14 parts of phoenix-tail fern, 8-13 parts of pine needle, 8-10 parts of hypericum sampsonii hance, 5-9 parts of concha arcae, 3-6 parts of paris polyphylla, 2-5 parts of grangea maderaspatana and 8-14 parts of oriental wormwood; the base component comprises the following components in parts by weight: MS, 0.10-0.19mg / L KT, 0.08-0.18mg / L GA3, 1.5-2.2mg / L MgSO4, 3.5-4.1mg / L NH4NO3, 10-20mg / L pomegranate bark juice and 110-200mg / L agar culture-medium; and the bacteriostatic stevia rebaudiana culture medium is used for tissue culture by taking roots, stems or leaves as explants. In order to overcome the defects of the bacteriostatic measures in the present stevia rebaudiana tissue culture, the Chinese herbal medicines are added into the culture medium and the culture medium is under an environment having a function of preventing and treating the microorganisms, such as, external bacteria all the time, so that the efficient and long-lasting bacteriostatic effect can be achieved, the investment in manpower and material resources can be reduced and the cost can be lowered.

Description

technical field [0001] The invention belongs to the technical field of stevia culture medium, and in particular relates to a bacteriostatic stevia culture medium. Background technique [0002] In the process of tissue culture, there are several common problems, namely microbial contamination, browning, vitrification and other phenomena. In recent years, the tissue culture technology of stevia rebaudiana has developed rapidly, and the problem of microbial contamination has become more and more prominent, and the research on this problem has also become more and more in-depth. [0003] The problem of microbial contamination mainly comes from two aspects: one is the contamination of the plant material itself; the other is the contamination caused by the operators and the microorganisms in the air; in addition, the contamination may also be caused by cross-contamination of the culture medium and operating tools. Contaminating microorganisms are divided into two categories: bact...

AI Technical Summary

Problems solved by technology

The above-mentioned measures to prevent microbial contamination in the tissue culture process all have certain effects, but the disadvantage is that each antibacterial link is isolated and cannot form a complete and unified antibacterial chain. If any link in the process is negligent, microorganisms will take advantage of it. , resulting in failure of tissue culture, reducing the quality and efficiency of tissue culture. In addition, through sterilization of the medium, aseptic inoculation, and cultivation in a sterile environment, the purpose of sterilization and antibacterial is achieved through external operations. In these links Once any link is not in place, bacteria and other microorganisms will be infected. Therefore, a measure of continuous antibacterial function is needed to keep the stevia explants in a sterile environment for a long time, and at the same time enhance the internal antibacterial function of stevia explants Ability to resist contamination by foreign microorganisms