Method for batch production of mat grass through one-step culture

A technology of mat grass and seedling batching, which is applied in the field of batch production of mat grass in one step, can solve the problems of quality decline and unsuitability for processing, and achieve the effects of short seedling delay period, saving rooting time, and improving excellent characteristics

Inactive Publication Date: 2017-05-10
INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Mat grass is a perennial herbaceous plant. Conventional breeding is carried out by division

Method used

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  • Method for batch production of mat grass through one-step culture
  • Method for batch production of mat grass through one-step culture
  • Method for batch production of mat grass through one-step culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1, tissue culture rapidly propagating mat grass

[0032] 1. Culture medium preparation and sterilization

[0033] Primary subculture medium for basic seedling propagation: 1 / 2MS+6-BA3.5mg / L;

[0034] One-step seedling mass production medium: 1 / 2MS+6-BA2.5mg / L+NAA0.6mg / L+IAA0.5mg / L+paclobutrazol 3.0mg / L.

[0035] The above medium was sterilized at 121°C for 20 minutes.

[0036] 2. Basic seedling propagation

[0037] Take the tiller nodes of mat grass (Juncus effusus L.), dig out the whole mat grass from the field, clean the roots, put them in clean water and cultivate them for about 2 weeks, then divide the tiller nodes into individual plants, and then grow the roots and base Leaves and tops are removed, tiller nodes are kept about 2cm high; when disinfecting, first clean them with washing powder, wash them with sterile water 5 to 7 times on a sterile operating table, soak them in 70% alcohol for 30 seconds to 1 minute, and then put Put it into 0.1% mercur...

Embodiment 2

[0044] Embodiment 2, tissue culture rapidly propagating mat grass

[0045] 1. Culture medium preparation and sterilization

[0046] Primary subculture medium: 1 / 2MS+6-BA4.0mg / L;

[0047] One-step seedling mass production medium: 1 / 2MS+6-BA3.0mg / L+NAA0.6mg / L+IAA0.4mg / L+paclobutrazol 4.0mg / L.

[0048] Get mat grass (Juncus effusus L.) tiller nodes, the basic operation method is consistent with embodiment 1; the effect is not much different from embodiment 1.

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Abstract

The invention discloses a method for batch production of mat grass through one-step culture. The method comprises the following steps of (1) acquiring basic seedlings: selecting tillering nodes of find mat grass plants as explants, sterilizing the explants, culturing the sterilized explants on 1/2MS+6-BA 1.2 to 4.5 mg/L of solid agar culture, and inducing the tillering nodes; (2) carrying out batch production: inoculating the tillering nodes acquired through the step (1) onto a solid agar culture with 1/2MS+6-BA 1.2 to 4.5 mg/L + NAA 0.02 to 1.0 mg/L + IAA 0.02 to 1.0mg/L + paclobutrazol 0.5 to 5.0mg/L for propagating and rooting; (3) carrying out field production: before taking seedlings out of a test-tube for transplanting, opening a test-tube cap for hardening the seedlings, taking out for cleaning the culture medium on the root part, and then directly transplanting the seedlings into a paddy field. The method is excellent in culture effect, high in propagation factor, good in stability and low in production cost, and is a fast propagation method for acquiring a large number of test-tube plantlets within the shortest time.

Description

technical field [0001] The invention belongs to the technical field of mat grass tissue culture, and in particular relates to a batch production method for mat grass seedlings in one step. Background technique [0002] Mat grass (Juncus effusus L.) is an important economic crop. It is soft and elastic, not easy to fade, and has the characteristics of moisture absorption, deodorization, insect resistance, moth resistance and natural grass fragrance, which makes mat grass very suitable for weaving tatami. , straw mats, straw mats, straw fans and straw baskets, etc., and their products have the functions of ventilation, moisture absorption and cooling. Because the mat grass can adjust the dryness and humidity by itself, the products made of mat grass can keep moderately dry in summer, which makes people's skin feel very comfortable, and has good thermal insulation performance in winter. Therefore, mat grass woven products have become an important part of people's daily life. H...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 黄丽芳夏新界
Owner INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI
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