Application of fermented products of discarded feathers in the preparation of feed for black soldier flies
A fermented product, black soldier fly technology, applied in the field of environmental resource utilization, can solve the problems of fermentation treatment of undiscovered feathers, achieve remarkable breeding effects, reduce nitrogen emissions, and low energy consumption
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[0037] Preparation of fermentation buffer: 0.5000g sodium chloride, 0.0600g anhydrous calcium chloride, 0.1000g magnesium chloride, 1.4000g dipotassium hydrogen phosphate, 0.7000g potassium dihydrogen phosphate, adjust the pH to 7.0, and distilled water to 1000mL.
[0038] Bacillus cereus (Bacillus cereus) YSQ08 was deposited in the China Center for Type Culture Collection on September 3, 2012, and the strain preservation number is CCTCC No.M2012322.
Embodiment 1
[0040] (1) Bacillus cereus YSQ08 (Bacillus cereus) was activated by streak culture on a plate, and a single colony was picked, placed in LB broth medium, and cultured on a shaking table at 37°C and 200r / min for 24h. Using the method of induction culture, add the activated bacteria solution to the feather-containing medium at 2% for 72 hours, then streak and invert for 14 hours, pick a single colony for liquid culture, and culture on a shaking table at 37°C and 200r / min for 14 hours .
[0041] The induced Bacillus cereus YSQ08 (Bacillus cereus) was added to the LB broth medium at an addition amount of 2% to carry out expanded culture for 3 times to obtain the activated bacterial liquid.
[0042] (2) Add 3% of the activated bacterium liquid induced in (1) to the fermentation buffer with a mass content of 3.5% of chicken feathers and a pH of 7. Keep the fermentation temperature at 37°C, and the fermentation time is 6 days.
[0043] (3) Pour the liquid in the Erlenmeyer flask in...
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