Crack self-healing recycled concrete based on urease-producing microbial mineralization deposition and its preparation method
A technology of recycled concrete and microorganisms, which is applied in the field of self-repairing recycled concrete materials for cracks and self-repairing recycled concrete for cracks.
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Embodiment 1
[0045] A crack self-healing recycled concrete based on the mineralization deposition of urease-producing microorganisms, which is made of the following raw materials in parts by weight:
[0046] 90 parts of expanded perlite particles loaded with urease-producing microorganisms and wrapped on the surface, 400 parts of cement, 562 parts of recycled coarse aggregate, 562 parts of natural stone, 475 parts of sand, 22 parts of silica fume, urease-producing microorganism suspension (concentration of 5.5×10 7 22 parts per mL), 193 parts of water, 8 parts of urea, 8 parts of calcium chloride, and 4 parts of superplasticizer.
[0047] The preparation method of the expanded perlite particles loaded with urease-producing microorganisms and wrapped on the surface comprises the following technical process:
[0048] Vector preparation:
[0049] (1) Select Bacillus pasteurianum, purify and cultivate the strain;
[0050] (2) Preparation of medium for Bacillus pasteurianus (content of each ...
Embodiment 2
[0066] A crack self-healing recycled concrete based on the mineralization deposition of urease-producing microorganisms, which is made of the following raw materials in parts by weight:
[0067] 100 parts of expanded perlite particles loaded with alkaliphilic urease bacteria and wrapped on the surface, 420 parts of cement, 575 parts of recycled coarse aggregate, 575 parts of natural stone, 500 parts of sand, 24 parts of silica fume, urease-producing microorganism suspension (concentration 5.5×10 7 23 parts per mL), 202 parts of water, 8 parts of urea, 8 parts of calcium chloride, and 4 parts of superplasticizer.
[0068] The preparation method is the same as in Example 1.
Embodiment 3
[0070] A crack self-repairing regeneration concrete based on the mineralized deposition of urease bacteria, which is made of the following raw materials in parts by weight:
[0071] 115 parts of expanded perlite particles loaded with alkaliphilic urease bacteria and wrapped on the surface, 450 parts of cement, 826 parts of recycled coarse aggregate, 354 parts of natural stone, 530 parts of sand, 26 parts of silica fume, urease-producing microorganism suspension (concentration 6.0×10 7 24 parts per mL), 216 parts of water, 9 parts of urea, 9 parts of calcium chloride, and 5 parts of superplasticizer.
[0072] The preparation method is the same as in Example 1.
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