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A kind of bacillus subtilis and application thereof

A technology of bacillus subtilis and its composition, applied in the field of bacillus subtilis and its application, can solve the problems of odor generation, long time for corpse treatment, etc.

Active Publication Date: 2020-05-08
福建洛东生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the use of biodegradation method to deal with animal carcasses, but the time to deal with the carcasses is too long, the process will produce foul smell

Method used

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  • A kind of bacillus subtilis and application thereof
  • A kind of bacillus subtilis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 Fermentation test of organic fertilizer

[0032]There were 2 control groups and 1 test group in this experiment. Each group composts 3 groups of organic fertilizers according to the weight ratio of 85% pig manure and 15% straw, and the control group 1 does not add any bacteria; the control group 2 adds 100g of Bacillus subtilis with the preservation number CCTCCM2016441 to each cubic compost raw material (The number of live bacteria is 1.0×10 10 CFU / g); The test group added the Bacillus subtilis 100g (viable bacteria number is 1.0×10 10 CFU / g) and the preservation number are Bacillus subtilis 100g of CCTCC M2016443 (the number of live bacteria is 1.0×10 9 CFU / g); 3 groups of organic fertilizers were mixed and piled up, and turned over every other day.

[0033] The fermentation time of control group 1 is 28 days, wherein the 3rd to 12th days are the high temperature period, and the temperature in the high temperature period is 58~70°C.

[0034] The ferme...

Embodiment 2

[0040] Example 2 Strawberry planting test

[0041] Two groups of greenhouse strawberries were selected, and the planting area of ​​each group of strawberries was 100 square meters. They were applied when the strawberries were planted and harvested after 6 months. The organic fertilizer fermented by the control group 2 in Example 1 was applied to the control group, and the organic fertilizer fermented by the test group in Example 1 was applied to the test group.

[0042] After 180 days of sowing, the strawberries were harvested. The yield per mu of strawberries in the control group was 430 kg, and the yield per mu of strawberries in the test group was 508 kg. The yield of strawberries in the test group was 18% higher than that of the control group. The test results show that the addition of Bacillus subtilis with the preservation number CCTCC M2016443 in the organic fertilizer heap can significantly increase the compost and soil fertilizer efficiency.

Embodiment 3

[0043] Embodiment 3 is used for the composition of biological fermentation bed

[0044] 100g preservation number is the Bacillus subtilis of CCTCC M2016441 (viable bacteria number is 1.0×10 10 cfu / g), 100g preservation number is the Bacillus subtilis of CCTCC M2016443 (the number of viable bacteria is 5.0×10 8 cfu / g), uniformly mixed with 800g zeolite powder to obtain 1000g biological fermentation bed composition, which will be expanded in the same proportion during production.

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PUM

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Abstract

The invention relates to a bacillus subtilis preserved in CCTCC with the preservation number of CCTCC M 2016443 and application thereof. The bacillus subtilis has strong enzyme-producing ability in a microaerobic environment, and the activity of lipase is strong. The bacillus subtilis can reduce the volatilization of ammonia in the fermentation of organic fertilizers and protect the fertilizer efficiency; in the application of deodorization and antibacterial in farms, it can reduce the number of coliforms, strongly inhibit harmful microorganisms in the environment, and adjust the environment Ecological balance is beneficial to the health of the breeding environment. In the application of harmless treatment of livestock and poultry carcasses, it can effectively decompose oil and reduce the emission of odor. In the application of biological fermentation bed, it can make the pig manure decompose more thoroughly, and reduce the harmful gas such as ammonia gas emitted.

Description

technical field [0001] The invention relates to the field of microorganism application, in particular to a bacillus subtilis and its application. Background technique [0002] Bacillus subtilis is a species of Bacillus. A single cell is 0.7-0.8×2-3 microns, evenly colored. Uncapsulated, with periosteum flagella, able to move. Gram-positive bacteria, spores 0.6-0.9×1.0-1.5 microns, elliptical to columnar, located in the center of the bacterium or slightly biased, the bacterium does not expand after spore formation. The surface of the colony is rough and opaque, stained white or yellowish, and often forms wrinkles when growing in liquid medium. Aerobic bacteria can use protein, various sugars and starch to decompose tryptophan to form indole. It is widely used in genetic research, and the synthesis pathway and regulation mechanism of purine nucleotides in this bacterium are relatively clear. Widely distributed in soil and decaying organic matter, it is easy to reproduce i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F15/00C05F11/08C12R1/125
CPCC05F3/00C05F11/08C12N1/205C12R2001/125C05F11/00
Inventor 刘明刚李坤卢祯鑫
Owner 福建洛东生物技术有限公司