Method of producing full thickness skin having skin accessory organs

A skin and organ technology, applied in the field of full-thickness skin with skin appendages, can solve the problems of inability to obtain test results, difficulty in obtaining test results, poor skin barrier function, etc., and achieve the effect of reducing the risk of causing tumors

Inactive Publication Date: 2017-05-31
ORGAN TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because there is no secretion of sebum or sweat, etc., the skin barrier function of the substitute skin without skin appendages is poor, and it is difficult to obtain test results close to those in living bodies
In addition, test results regarding the effects of cosmetics or pharmaceuticals on the skin appendages themselves cannot be obtained using skin substitutes that do not have the skin appendages

Method used

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  • Method of producing full thickness skin having skin accessory organs
  • Method of producing full thickness skin having skin accessory organs
  • Method of producing full thickness skin having skin accessory organs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0079] Formation of full-thickness skin by in vivo transplantation of conjugates containing embryoid bodies

[0080] 1. Materials and Methods

[0081] (1) Experimental animals

[0082] C. B-17 / lcr-scid / scidJcl mice were purchased from CLEA (Tokyo, Japan), and scid / scid hr / hr (SHO) mice were purchased from Charles River (Kanagawa, Japan). Mice were managed and handled in accordance with NIH Laboratory Animal Guidelines. All experiments were performed under the approval of the Experimental Animal Control Committee of Tokyo University of Science.

[0083] (2) Cell culture

[0084] Mouse iPS cells (mGF-iPS-3F-3) were co-cultured with SNLP76.7-4 feeder cells treated with mitomycin C (Nacalai Tesque). For culture medium, supplemented with 15% fetal bovine serum (Japan BioSerum), 50 units / ml penicillin, 50 μg / ml streptomycin, 2 mM L-glutamine, 1×10 -4 M 2-mercaptoethanol and 1 x 10 -4 M Dulbecco's Modified Eagle's Medium (DMEM without sodium pyruvate; Nacalai Tesque) for no...

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PUM

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Abstract

The invention provides a method of efficiently producing full thickness skin having skin accessory organs. [Solution] This method of producing full thickness skin having skin accessory organs is characterized by involving the following steps (a) to (d): (a) a step for stimulating an embryoid body with a physiologically active substance that can activate the Wnt pathway, (b) a step for preparing a conjugate that includes all or part of the embryoid body stimulated in step (a) and a scaffolding material, (c) a step for transplanting the conjugate prepared in step (b) into an animal, and (d) a step for producing, in the animal, full thickness skin derived from said conjugate.

Description

technical field [0001] The present invention relates to a method for producing full-thickness skin (full-thickness skin) with cutaneous appendages (skin appendages) and to the produced full-thickness skin with cutaneous appendages. Background technique [0002] Pharmacology / safety tests are performed using mice and rats, etc. in the development of cosmetics and dermatological drugs. However, in recent years, the development of alternative methods to animal experiments has been searched worldwide in consideration of animal welfare. [0003] Although alternative testing methods using cultured cells or cell sheets (cell syring) simulating the epidermis or dermis of animals have been developed as an alternative to animal experiments, these surrogate skins do not include the skin appendages that animal skin has (such as hair follicles, nails, sebaceous glands, sweat glands, and mammary glands). Since there is no secretion of sebum or sweat or the like, the skin barrier function...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/00C12N5/071
CPCA61L27/00A01K67/0271A61L27/36A61L27/3604A61L27/38A61L27/40A61L27/60C12N5/0696A01K2207/10A01K2207/12A01K2227/105A01K2267/025A01K2267/0331C12N2501/415C12N2502/1323A61K9/0014C12N5/0603C12N5/0629C12N2509/00G01N33/5008G01N33/5073
Inventor 辻孝丰岛公荣
Owner ORGAN TECH
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