Preparation method of cross-linked sodium hyaluronate stem cell gel for uterine cavity

Abstract

The invention discloses a preparation method of cross-linked sodium hyaluronate stem cell gel for a uterine cavity. The preparation method comprises the five steps: preparing cross-linked sodium hyaluronate gel, separating amniotic mesenchymal stem cells, purifying and culturing the amniotic mesenchymal stem cells, extracting the amniotic mesenchymal stem cells and mixing and preparing the cross-linked sodium hyaluronate stem cell gel. The hAMSCs which are wide in source, free of ethical limitation, low in immunogenicity and free of tumorigenic risk are selected as stem cell components, the hAMSCs are mixed with the sodium hyaluronate gel to enhance the adhesion and fusion characteristics of the hAMSCs, a good biological function is achieved, the sodium hyaluronate gel has adhesion, a net-shaped structure can be formed in an aqueous solution, and an endometrial wound surface is separated, so as to play the role of mechanical barrier and space barrier; and meanwhile, the material source, ethical permission, experimental operation, expected results and the like are fully guaranteed, and conversion, large-scale production and subsequent clinical use of products are greatly facilitated.

Description

technical field [0001] The invention relates to the technical field of IUA adhesion prevention, in particular to a preparation method of cross-linked sodium hyaluronate stem cell gel for uterine cavity. Background technique [0002] Intrauterine adhesion (IUA) refers to the damage and scar healing of the endometrial basal layer due to injury or infection, resulting in partial or complete adhesion of the uterine cavity, uterine isthmus, and cervical canal wall, causing uterine cavity shrinkage. IUA has become one of the important causes of female infertility in recent years, accounting for about 8% of the pathogenesis of infertility, and it is also associated with recurrent Miscarriage, fetal growth restriction, repeated embryo implantation failure, abnormal placental implantation site, etc. are closely related; [0003] Commonly used IUA adhesion prevention methods include intrauterine placement of balloons or IUDs, intrauterine injection of sodium hyaluronate and other bio...

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Problems solved by technology

[0003] Commonly used IUA adhesion prevention methods include intrauterine placement of balloons or IUDs, intrauterine injection of sodium hyaluronate and other biological glues, intrauterine transplantation of stem cells or human amniotic membrane, etc., as well as estrogen drugs, but The barrier area of ​​conventional birth control ring is limited and there is a risk of incarceration, so the prevention effect is not good; the balloon may compress the uterine cavity, affect the blood supply and repair process of the endometrium, and affect the function of the cervix; the use of biological glue such as sodium hyaluronate alone cannot significantly improve Pregnancy outcome of the patient; estrogen drugs cannot physically block the front and rear walls of the uterine cavity, and there are certain contraindications and risks of thrombosis, so the prevention of IUA and the problem of postoperative re-adhesion have not been fundamentally improved. The present invention proposes a Preparation method of cross-linked sodium hyaluronate stem cell gel for uterine cavity to solve problems in the prior art

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