Method for controlling incubator to be applicable to induction and amplification of immune cells

An immune cell and cell technology, applied in the direction of blood/immune system cells, animal cells, tissue culture, etc., can solve the problems of limited application of cellular immunotherapy, poor uniformity of immune cells, and high probability of tumorigenesis, and achieve the efficiency of induction differentiation The effect of improvement, uniformity improvement, and amplification efficiency improvement

Active Publication Date: 2018-05-22
广州沙艾生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, in the prior art, human autoimmune cells obtained through in vitro culture have the problems of low expansion efficiency, poor uniformity of immune cells, reinfusion into patients, and high probability of tumorigenesis.
Applications of cellular immunotherapy therapies are limited

Method used

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  • Method for controlling incubator to be applicable to induction and amplification of immune cells
  • Method for controlling incubator to be applicable to induction and amplification of immune cells
  • Method for controlling incubator to be applicable to induction and amplification of immune cells

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Embodiment 1

[0030] The following will introduce in detail the method of using the incubator according to the embodiment of the present invention, so that the incubator operates in the control method according to the embodiment of the present invention, and the method for inducing the expansion of immune cells, the method includes inducing isolated mononuclear cells Expand into immune cells, and detect the activity of immune cells.

[0031] 1. Experimental method

[0032] 1. Preparation of Anti-human CD16 Coated ZellWerk Bioreactors

[0033] 1.1 Add 15 mL of 2.5 μg / mL anti-human CD16 monoclonal antibody dissolved in medical normal saline to the bioreactor, shake the culture bottle gently to make the antibody cover the culture surface, and overnight at 4°C in the dark.

[0034] 1.2 Recover the antibody coating solution before use, wash the bioreactor once with 15mL of normal saline, and then use 15mL of T cell expansion medium (OpTmizer TM CTS TM T-cell expansion SFM) and washed once.

...

Embodiment 2

[0079] 1. Experimental method

[0080] 1. Preparation of Anti-human CD16 Coated ZellWerk Bioreactors

[0081] 1.1 Add 15 mL of 2.5 μg / mL anti-human CD16 monoclonal antibody dissolved in medical normal saline to the bioreactor, shake the culture bottle gently to make the antibody cover the culture surface, and overnight at 4°C in the dark.

[0082] 1.2 Recover the antibody coating solution before use, wash the bioreactor once with 15mL of normal saline, and then use 15mL of T cell expansion medium (OpTmizer TM CTS TM T-cell expansion SFM) and washed once.

[0083] 2. Collect peripheral blood, separate peripheral blood plasma and mononuclear cells

[0084] 2.1 Collect about 100ml of human peripheral blood with a sterile blood collection bag added with anticoagulant, and reserve 1ml of peripheral blood for quick test and blood type identification. After passing the quick test, the peripheral blood will be sent to the GMP laboratory. If the quick test fails, the blood sample w...

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Abstract

The invention provides a method for controlling an incubator to be applicable to induction and amplification of immune cells. The method comprises the steps: a controller starts an oxygen source and acarbon dioxide source for supplying oxygen and carbon dioxide into a culturing space; the controller starts an oxygen concentration sensor and a carbon dioxide concentration sensor and acquires dataincluding oxygen concentration and carbon dioxide concentration in the space.

Description

technical field [0001] The invention relates to the field of biology, and in particular, the invention relates to a method for controlling an incubator suitable for inducing expansion of immune cells. Background technique [0002] Cellular immunotherapy is to collect the body's own immune cells, culture them in vitro, increase their number by thousands of times, and enhance their targeted killing function, and then infuse them back into the human body to kill pathogens, cancer cells, The mutated cells break immune tolerance, activate and enhance the body's immune ability, and take into account the dual effects of treatment and health care. [0003] However, in the prior art, there are problems such as low expansion efficiency and poor uniformity of immune cells obtained by culturing the obtained human immune cells in vitro, and high probability of tumorigenesis when reinfused into patients. The application of cellular immunotherapy therapy is limited. [0004] Therefore, h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0786C12N5/0783C12M3/00C12M1/36C12M1/34C12M1/04
CPCC12M29/06C12M41/34C12N5/0636C12N5/0645C12N2501/2302C12N2501/999C12N2533/50
Inventor 徐智峰张新
Owner 广州沙艾生物科技有限公司
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