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Method of detecting a microorganism using chromatographic enrichment

A kind of microorganism, a part of technology, applied in certain fields, can solve problems such as existing needs

Inactive Publication Date: 2017-05-31
3M INNOVATIVE PROPERTIES CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Despite advances in sample preparation, there remains a need for methods to quickly and easily prepare samples for detection of target cells

Method used

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  • Method of detecting a microorganism using chromatographic enrichment
  • Method of detecting a microorganism using chromatographic enrichment
  • Method of detecting a microorganism using chromatographic enrichment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach A

[0108] Embodiment A is a method for detecting target cells, comprising:

[0109] A liquid sample is brought into contact with a porous carrier of a sample preparation device comprising:

[0110] a housing including a first opening and a second opening; and

[0111] porous carrier;

[0112] wherein the porous carrier includes a sample receiving region at a first predetermined location and a target cell binding region at a second predetermined location, the porous carrier defining a first fluid pathway extending from the sample receiving region through the target cell binding region;

[0113] wherein at least a portion of the porous carrier is disposed within the housing;

[0114] wherein the second fluid pathway extends through the housing from the first opening to the second opening, the second fluid pathway intersecting the porous carrier at the target cell binding zone;

[0115] moving at least a portion of the liquid sample longitudinally through the porous carrier from ...

Embodiment approach D

[0120] Embodiment D is a method for detecting target cells

[0121] A liquid sample is brought into contact with a porous carrier of a sample preparation device comprising:

[0122] a housing including a first opening and a second opening; and

[0123] porous carrier;

[0124] wherein the porous carrier includes a sample receiving region at a first predetermined location and a target cell binding region at a second predetermined location, the porous carrier defining a first fluid pathway extending from the sample receiving region through the target cell binding region;

[0125] wherein at least a portion of the porous carrier is disposed within the housing;

[0126] wherein a second fluid pathway extends through the housing from the first opening and the second opening, the second fluid pathway intersecting the porous carrier at the target cell binding zone;

[0127] moving at least a portion of the liquid sample longitudinally through the porous carrier from the sample rec...

Embodiment approach L

[0137] Embodiment L is a kind of device, comprises:

[0138] a housing including an interior, a first opening and a second opening; and

[0139] a porous carrier comprising a sample receiving area and a target cell binding area;

[0140] wherein the porous carrier defines a first fluid pathway extending from the sample receiving zone to the target cell binding zone;

[0141] wherein at least a portion of the porous carrier is disposed inside the housing;

[0142] wherein a second fluid pathway including a central axis extends through the housing from the first opening and the second opening, the second fluid pathway intersecting the porous carrier at the target cell binding zone;

[0143] wherein the central axis is oriented orthogonally to the first fluid passage.

[0144] Embodiment M is the device of embodiment L, further comprising a sample port accessible to the sample receiving area, wherein the sample receiving area is disposed within the interior of the housing.

...

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Abstract

A device is provided. The device comprises a casing comprising an interior, a first opening, and a second opening; and a porous carrier comprising a sample-receiving zone and a target cell-binding zone. The porous carrier defines a first fluid pathway that extends from the sample-receiving zone to the target cell-binding zone. At least portion of the porous carrier is disposed in the interior of the casing. A second fluid pathway comprising a central axis extends through the casing from the first opening and the second opening, the second fluid pathway intersecting the porous carrier at the target cell-binding zone. The central axis is oriented orthogonally with respect to the porous carrier. Methods of using the device to detect a target microorganism are also provided.

Description

[0001] Cross References to Related Applications [0002] This patent application claims priority to US Provisional Patent Application No. 62 / 060,621, filed October 7, 2014, the disclosure of which is incorporated herein by reference in its entirety. Background technique [0003] In some cases, detection of specific cells (eg, microorganisms) in complex matrices (eg, food, wound effluent, saliva, sputum, feces) may be difficult because the matrix contains one or more components of the detection system Interfering substances (eg, biomolecules, enzymes, chemicals, ions). Therefore, operators typically use various methods to reduce the inhibitory effects of substances found in the matrix. One way to reduce matrix effects is to dilute matrix-containing samples to reduce the concentration of inhibitory substances. However, this approach can limit the overall detection sensitivity of the test. [0004] Another approach to reduce matrix effects in samples is to use immunomagnetic ...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/569
CPCG01N33/558G01N33/56911G01N2469/10G01N33/54388
Inventor 尼尔·帕西格雷戈里·W·西顿
Owner 3M INNOVATIVE PROPERTIES CO