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Composition for improving skin

A composition and skin aging technology, applied in the direction of skin care preparations, skin diseases, drug combinations, etc., can solve the problems of weak moisturizing effect of wrinkles, skin irritation and redness, and fragility of component changes, so as to prevent skin wrinkles, improve or Aging, skin wrinkle improvement effect

Inactive Publication Date: 2017-06-16
LG HOUSEHOLD & HEALTH CARE LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, products containing these materials are not only vulnerable to changes in product ingredients over time, but also may cause significant skin irritation, redness, etc. The problem, therefore, is that its usage is limited for safety, so that the wrinkle prevention and improvement effects and moisturizing effects are substantially weak

Method used

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  • Composition for improving skin
  • Composition for improving skin
  • Composition for improving skin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0097] Example 1: Confirmation of the effect of promoting the synthesis of collagen type I in human fibroblasts

[0098] When fisetin was added to the culture medium of human fibroblasts, the effect of promoting collagen synthesis was confirmed at the cellular level. The fisetin used was purchased from Sigma Aldrich (USA). The synthetic collagen was quantified using a PICP EIA kit (Procollagen Type I C-Peptide Enzyme Immuno Assay KIT).

[0099] In the experiment, the final concentration of fisetin was adjusted to 10 μg / mL, 20 μg / mL. After adding fisetin to the culture medium (DMEM medium) of human fibroblasts and culturing for 48 hours, take the culture solution, pass through the PICP EIA kit, and use a spectrophotometer to measure the concentration of fisetin in each concentration at 450nm. The degree of collagen synthesis.

[0100] In order to compare the effect, the degree of collagen synthesis was measured by the same method for the fibroblast culture medium to which no...

Embodiment 2

[0104] Example 2: Confirmation of the effect of promoting the synthesis of sulfated glycosaminoglycans in human fibroblasts

[0105] When fisetin was added to the culture medium of human fibroblasts, the effect of promoting the synthesis of sulfated glycosaminoglycans was confirmed at the cellular level. The determination of sulfated glycosaminoglycans was quantified using a sulfated aminoglycan assay kit (Blyscan glycosaminoglycan assay kit, Biocolor).

[0106] In the experiment, the final concentration of fisetin was adjusted to 1 μg / mL, 10 μg / mL, 20 μg / mL. After adding fisetin to the culture medium (DMEM medium) of human fibroblasts and culturing for 24 hours, the culture solution was taken, passed through the aminoglycan sulfate detection kit, and using a spectrophotometer, measured the respective concentrations at 656 nm. The total amount of sulfated glycosaminoglycans in the concentration.

[0107] The increase rate of sulfated glycosaminoglycan production was calculat...

Embodiment 3

[0111] Example 3: Increase of fisetin-based hyaluronan synthase (HSS) gene expression in human keratinocytes

[0112] Human keratinocytes were cultured in DMEM medium containing 10% bovine serum (fetal bovin serum) at 1×10 6 Cells / mL were cultured, respectively injected into 6-well plates, with an injection volume of 3000 μL per well, and cultured for 18 hours. Then, it was washed twice with DMEM medium not containing bovine serum, and treated with fisetin (1 ppm or 10 ppm) for 24 hours. The cells were recovered, and all RNA was extracted by RNeasy mini kit (Qiagen), and then quantified, and 1 μg of RNA was used RNA PCR Kit (Applied Biosystems) was used for reverse transcription. The reverse transcription reaction is carried out by PCR instrument (Biorad) was performed.

[0113] Then, using reverse transcription reaction solution 2 μ L and HAS-2, HAS-3, GAPDH (3-phosphate glyceraldehyde dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase) The probes (Invitrogen, US...

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PUM

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Abstract

The invention relates to a composition comprising fisetin as an active ingredient. The composition of the invention has an effect of improving or preventing skin aging, wrinkles, decreased elasticity, and decreased moisture content, and specifically, has the effect of fundamentally improving and preventing the wrinkles, aging, decreased elasticity, etc. of the skin by promoting the synthesis of collagen (type I) and sulfated glycosaminoglycans in the skin. In addition, since the composition is derived from a natural substance, the composition has low toxicity and few side effects and can be very effectively used as active ingredients in cosmetics, pharmaceuticals or foods, etc.

Description

technical field [0001] The invention relates to a cosmetic material for improving skin, a health-care functional food and a pharmaceutical composition. Background technique [0002] The skin is an important organ that undertakes the function of protecting the human body from the external environment. For example, it undertakes a variety of physiological functions such as a barrier function to prevent internal water and active ingredients from flowing out, a thermoregulation function, and an excretion function. As individuals age, the skin also loses important functions due to aging. The causes of skin aging can be roughly divided into: endogenous factors interpreted as aging; and exogenous factors due to sun damage and the like. Due to these internal and external factors, the skin ages, resulting in the formation of wrinkles and a decrease in the elasticity of the skin, which is mainly caused by damage and changes in the dermis. [0003] The structure of the skin is broad...

Claims

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Application Information

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IPC IPC(8): A61K8/49A61Q19/08A61K31/352A61P17/00A23L33/10
CPCA23V2002/00A61K8/498A61K31/352A61Q19/08A23V2200/30A23V2200/318
Inventor 李惠珍金美善李相和
Owner LG HOUSEHOLD & HEALTH CARE LTD
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