Vickers and aeromonas hydrophila bicombinant inactivated vaccine and large-scale preparation technology

A technology of Aeromonas hydrophila and dual inactivated vaccines, applied in vaccines, multivalent vaccines, veterinary vaccines, etc., can solve the problems of lack of large-scale preparation methods of vaccines, increase fishermen's income, and reduce water pollution , the effect of improving the effect of immunity

Inactive Publication Date: 2017-07-04
PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research and development of aquatic vaccines in China has progressed rapidly in the past ten years, but the large-scale preparation methods of vaccines are relatively lacking, which are basically laboratory preparation technologies. At present, there is an urgent need for aquatic vaccine production technologies that can be industrialized.

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  • Vickers and aeromonas hydrophila bicombinant inactivated vaccine and large-scale preparation technology
  • Vickers and aeromonas hydrophila bicombinant inactivated vaccine and large-scale preparation technology

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Embodiment Construction

[0015] The technical solutions of the present invention will be described in detail below through specific examples, but the scope of the present invention is not limited by these examples.

[0016] This embodiment provides a large-scale preparation technology of Vickers and Aeromonas hydrophila dual inactivated vaccine, including the following steps:

[0017] 1. Preparation of primary seed solution

[0018] Take out the strains (Aeromonas victorii and Aeromonas hydrophila) stored in the refrigerator, inoculate them on nutrient agar (see the appendix for the medium formula), cultivate them at 28°C for 24 hours, pick the moss and inoculate the nutrient agar at 28°C Cultivate for 24 hours, inoculate 300ml / bottle of nutrient broth (see the appendix for the medium formula), 10 bottles, place on a shaker at 200r / min, 28°C, and cultivate for 18 hours to obtain 3L of first-class seed liquid.

[0019] The strains selected in this example are: Aeromonas verkisii strain LYK090702 isola...

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Abstract

The invention relates to a bicombinant inactivated vaccine for immunological control of freshwater fish bacterial septicemia and a large-scale preparation technology. According to the invention, diseases fish body can be separated to obtain strong toxic strain, inactivated vaccine is prepared by large-scale production, immunization inoculation is carried out by injection and immersion, immunization is carried out on the fresh water cultured fish such as crucian and grass carp, and good diseases prevention effect is achieved; the disadvantages of single inactivated vaccine aiming at one pathogen is overcome, the provided bicombinant vaccine resists infection of two pathogens by one time application, immunization effect is increased, and vaccine can be conveniently used by immersion, injection or feed mixing for feeding.

Description

technical field [0001] The invention belongs to the technical field of immune prevention and treatment of aquaculture diseases, and in particular relates to a dual inactivated vaccine for the prevention and control of bacterial sepsis in freshwater fish and a large-scale preparation technology. Background technique [0002] Bacterial hemorrhagic disease of freshwater fish, also known as bacterial hemorrhagic septicemia, is one of the most serious infectious diseases that harm freshwater farmed fish. The fish that are harmed mainly include grass, silver carp, bighead carp, crucian carp, carp, bream, dace, eel, mandarin fish and other fish, causing serious losses (reaching more than 30%) to the freshwater aquaculture industry. The disease pathogen is mainly Aeromonas genus Aeromonas victoria) and Aeromonas hydrophila, and the bacterial septicemia disease that they cause accounts for more than 80%, is the modal pathogenic bacterium of freshwater fish. Generally, disinfectants ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/116A61K39/02A61P31/04A61P37/04
CPCA61K39/0208A61K2039/521A61K2039/552A61K2039/70
Inventor 陶家发赖迎迢任燕孙承文江小燕罗霞赵长臣巩华刘春花黄志斌
Owner PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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