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Sea cucumber polypeptide, and preparation method and application thereof

A technology of sea cucumber polypeptide and sea cucumber, which is applied in the field of marine biology, can solve problems such as oxidative stress, aging organ function, injury, etc., and achieve good anti-oxidation, wide application range, good economic and social effects

Inactive Publication Date: 2017-07-04
INFINITUS (CHINA) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The free radical theory of aging holds that under normal circumstances, the production and removal of free radicals in the body are in a state of dynamic balance, but under the influence of harmful factors in vivo and in vitro, diseases and aging states, endogenous active oxygen free radicals are continuously produced and the scavenging ability Significantly decreased, causing reactive oxygen species to damage cellular proteins, lipids, and DNA, resulting in oxidative stress, and eventually causing aging and organ function decline

Method used

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  • Sea cucumber polypeptide, and preparation method and application thereof
  • Sea cucumber polypeptide, and preparation method and application thereof
  • Sea cucumber polypeptide, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Embodiment 1, the preparation of sea cucumber polypeptide

[0055] Fresh sea cucumbers were dissected, viscera removed, body wall washed 3-4 times with tap water and distilled water, then homogenized sea cucumber body wall tissue material, freeze-dried, weighed 5g into a 250mL beaker and added 80mL water to infiltrate (1g dry powder mixed with 20mL of first-class water, to be replenished with enzyme solution later), can be stirred properly with a glass rod, then preheated to 45°C in a water bath, adjusted to pH 8.0 with NaOH solution, and the trypsin activity is 2500U / mg, expressed as per gram Add 3000U of substrate to trypsin, hydrolyze at 37-55°C for 2-10 hours at a constant temperature, and inactivate by boiling at 100°C; then, when the temperature drops to about 55°C, adjust the pH to 5.5 with 0.1M HCl solution, papaya The protease activity is 6000U / mg, add papain in the amount of 3000U per gram of substrate, hydrolyze at 50-80°C for 2-10 hours at a constant tempera...

Embodiment 2

[0056] Embodiment 2, the preparation of sea cucumber polypeptide

[0057] Fresh sea cucumbers were dissected, viscera removed, body wall washed 3-4 times with tap water and distilled water, then homogenized sea cucumber body wall tissue material, freeze-dried, weighed 5g into a 250mL beaker and added 80mL water to infiltrate (1g dry powder mixed with 20mL of first-class water, to be replenished with enzyme solution later), can be stirred properly with a glass rod, then preheated to 45°C in a water bath, adjusted to pH 8.0 with NaOH solution, and the trypsin activity is 2500U / mg, expressed as per gram Add 3000U of substrate to trypsin, hydrolyze at 37-55°C for 2-10 hours at a constant temperature, and inactivate by boiling at 100°C; then, when the temperature drops to about 55°C, adjust the pH to 5.5 with 0.1M HCl solution, papaya The protease activity is 6000U / mg, add papain in the amount of 3000U per gram of substrate, hydrolyze at 50-80°C for 2-10 hours at a constant tempera...

Embodiment 3

[0059] Example 3, Anti-paraquat-induced oxidative stress experiment

[0060] Add the sea cucumber polypeptide prepared in Example 1 to wild-type C. elegans at the early adult stage at concentrations of 0.5 mg / mL and 2 mg / mL, and add the sea cucumber polypeptide component with a molecular weight less than 3 kDa prepared in Example 2 at a concentration of 1 mg / mL and 2 mg / mL were added to the wild-type C. elegans in the early adult stage, and the control group was added with an equal volume of S Medium. After culturing at 20°C for 24 hours, paraquat with a final concentration of 70 mM was added to model the oxidative stress of C. elegans. And start counting the survival rate of C. elegans regularly until all the worms die. The survival rate is represented by a survival curve, and Kaplan-meier method is used to analyze and compare the differences between the sea cucumber polypeptides prepared in Example 1 and Example 2 and the control group. The result is as figure 1 with fig...

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Abstract

The invention specifically relates to a sea cucumber polypeptide and a preparation method and application thereof, belonging to the field of marine biotechnology. The preparation method for the sea cucumber polypeptide comprises the following steps: subjecting the body wall of a sea cucumber to homogenization, then carrying out hydrolysis with trypsin and papain successively, adding ethanol for precipitation of polysaccharides, carrying out centrifugation, taking supernatant, and intercepting components with a molecular weight of less than 3 kDa from the supernatant so as to obtain sea cucumber polypeptide components with a molecular weight of less than 3 kDa. Experimental results show that the sea cucumber polypeptide and the sea cucumber polypeptide components with a molecular weight of less than 3 kDa prepared by the invention have good anti-oxidation and anti-ageing effect, can be used for preparation of anti-oxidation and anti-ageing drugs or health products, and have good economic and social effect.

Description

technical field [0001] The invention belongs to the technical field of marine biology, and in particular relates to a sea cucumber polypeptide and its preparation method and application. Background technique [0002] The aging of the global population is becoming more and more serious. Aging and aging-related diseases have become an important factor threatening human death, and have attracted widespread attention in the field of medical care at home and abroad. The mechanism of aging is extremely complex, and there are dozens of related theories, and the "oxidative free radical theory of aging" is related to other theories to a certain extent, and it is also one of the most widely accepted theories of aging. The free radical theory of aging holds that under normal circumstances, the production and removal of free radicals in the body are in a state of dynamic balance, but under the influence of harmful factors in vivo and in vitro, diseases and aging states, endogenous activ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06A61K38/01A61P39/06A23L33/18
CPCA61K38/012A61Q19/08A61K8/64A61K8/987A61K35/616C12P21/06
Inventor 郭晓蕾梁明马忠华张婷丁刘刚秦垂新毛新亮
Owner INFINITUS (CHINA) CO LTD
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