The invention relates to the technical field of eye masks, in particular to eye-brightening and glowing stay-up eye mask essence and a preparation method thereof. The eye-brightening and glowing stay-up eye mask essence is prepared from the following raw materials of deionized water, a component A, a component B, a component C, a component D and a component E, wherein the component A comprises 1,3-propylene glycol, allantoin, trehalose, acetyl chitosamine, sclerotium gum, and disodium EDTA, the component B comprises butylene glycol and hydrolyzed sodium hyaluronate, the component C comprises nicotinamide and magnesium ascorbyl phosphate, the component D comprises olea europaea fruit oil and a solubilizer, and the component E comprises a palmitoyl hexapeptide compound conditioner, an acetyltetrapeptide compound conditioner, a carnosine compound conditioner, a gentiana lutea root extract conditioner, a cornus officinalis fruit extract conditioner, a saccharomyces lysate extract conditioner, a herba centellae conditioner, a chlorella extract conditioner, a glycerol caprylate compound humectant, and an ethylhexylglycerin compound humectant. The eye mask essence is prepared in gradientstirring and temperature decreasing modes, full and even mixing of raw materials is promoted, biological activity of the raw materials is also ensured, and aging of periorbital skin can be effectively relieved.