Analytical method for identifying Chinese date honey and syrup-adulterated with fake Chinese date honey

Abstract

The invention discloses an analytical method for identifying Chinese date honey and syrup-adulterated with fake Chinese date honey, and particularly relates to a method by applying the ultra-high performance liquid chromatography-quadrupole rod-orbitrap high resolution mass spectrum technique combining metabonomics. The method comprises the steps that firstly, a true Chinese date honey sample and a syrup-adulterated with fake Chinese date honey sample to be detected are pretreated by organic solvent respectively, separation and determination of chemical components in the pretreated samples are achieved by applying an ultra-high performance liquid chromatography-quadrupole rod-orbitrap high resolution mass spectrometry method, then obtained UHPLC-MS original data of the true Chinese date honey sample and the Chinese date honey sample to be detected is preprocessed, and finally true Chinese date honey and syrup-adulterated with fake Chinese date honey are distinguished by applying principal component analysis of a multivariate statistical analysis method. According to the analytical method, after metabolin information of Chinese date honey and syrup-adulterated with fake Chinese date honey is obtained comprehensively, combined with multivariate statistical analysis, Chinese date honey and syrup-adulterated with fake Chinese date honey are analyzed comprehensively, and the detection of syrup-adulterated with fake Chinese date honey is completed.

Description

technical field [0001] The invention belongs to the technical field of food adulteration identification, and in particular relates to an analysis method for identifying jujube nectar and syrup adulterated jujube nectar using a metabolomics technology combined with UHPLC-Q Exactive. Background technique [0002] Honey is a natural sweet substance that bees collect nectar, secretions or honeydew from plants, mix with their own secretions, and fully brew. Honey has high nutritional value, but due to the high price and low output of honey, it makes Honey has become a main object of adulteration by illegal traders. In order to improve the production enthusiasm of beekeepers, protect the interests of consumers, support the fair competition of formal honey production enterprises, maintain the order of the honey market, and promote the healthy development of my country's honey industry, therefore It is of great significance and value to find the marker metabolites of real honey sample...

AI Technical Summary

Problems solved by technology

The sensitivity of nuclear magnetic resonance (NMR) is low, and the metabolites we are concerned about in honey may be ignored, and NMR equipment is expensive and not suitable for daily monitoring

Near-infrared spectroscopy (NIRS) also has its fatal shortcomings: 1. It requires a large number of representative samples with known chemical values ​​to build models

Thus, the analysis of small batches of samples using near-infrared becomes impractical

2. The model needs to be updated continuously. Due to the change of the instrument state or the change of the standard sample, the model will also change accordingly.

3. The model is not universal, and the model of each instrument is different, which increases the limitation of use

4. The cost of modeling is high and the cost of testing is high

The various syrups currently adulterated with honey are very similar to honey in terms of physical and chemical properties, composition and content, and flavor. Therefore, the above-mentioned methods for measuring the quality of honey cannot detect honey adulterated with syrup

[0005] In summary, there is a lack of a fast, effective and intuitive method for jujube nectar adulteration identification in the current prior art, and there is no combination of ultra-high performance liquid chromatography-quadrupole-orbitrap high-resolution mass spectrometry combined with metabolomics. Studies on Determination of Marker Metabolites in Jujube Honey by Scientific Method

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