Analytical method for identifying Chinese date honey and syrup-adulterated with fake Chinese date honey

An analysis method and a technology for jujube nectar, which are applied in the field of food adulteration identification, can solve the problems of high modeling cost, low sensitivity, increased use limitations, etc. Effect

Active Publication Date: 2017-07-07
SHANDONG NORMAL UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

The sensitivity of nuclear magnetic resonance (NMR) is low, and the metabolites we are concerned about in honey may be ignored, and NMR equipment is expensive and not suitable for daily monitoring
Near-infrared spectroscopy (NIRS) also has its fatal shortcomings: 1. It requires a large number of representative samples with known chemical values ​​to build models
Thus, the analysis of small batches of samples using near-infrared becomes impractical
2. The model needs to be updated continuously. Due to the change of the instrument state or the change of the standard sample, the model will also change accordingly.
3. The model is not universal, and the model of each instrument is different, which increases the limitation of u...

Method used

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  • Analytical method for identifying Chinese date honey and syrup-adulterated with fake Chinese date honey
  • Analytical method for identifying Chinese date honey and syrup-adulterated with fake Chinese date honey
  • Analytical method for identifying Chinese date honey and syrup-adulterated with fake Chinese date honey

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Effect test

Embodiment 1

[0075] (1) method principle of the present invention:

[0076] After the samples of real jujube nectar and syrup adulterated jujube nectar were treated with the same pretreatment method, UHPLC was used in series with Thermo Fisher Q Exactive Mass Spectrometer to realize the separation and determination of the chemical components in the samples, and Compounds Discoverer software was used to obtain the original The data were extracted from chromatographic peaks, peak alignment and library search analysis of unknown compounds. Multivariate statistical analysis and PCA analysis were used to distinguish real jujube nectar from syrup adulterated jujube nectar.

[0077] (2) Sample pretreatment

[0078] Weigh 1g jujube nectar sample (the number of repetitions for one sample is 6 times) in a 50mL centrifuge tube, add 20mL extractant (extractant is mixed with methanol containing 1% formic acid and ultrapure water in equal volume), vortex mix until jujube The nectar sample was complete...

Embodiment 2

[0093] The method for screening jujube nectar marker metabolites based on UHPLC tandem Thermo Fisher Q Exactive Mass Spectrometer combined with metabolomics method comprises the following steps:

[0094] (1) Sample pretreatment

[0095] Put 1g jujube nectar sample in a 50mL centrifuge tube, add 20mL extractant (the extractant is methanol containing 1% formic acid mixed with ultrapure water in equal volume), vortex mix until the jujube nectar sample is completely dissolved, and place the centrifuge tube in ultrasonic Sonicate in a washing machine for 25 minutes, centrifuge at 1000 rpm for 5 minutes, and filter the supernatant with a 0.22 μm organic filter membrane for sample loading.

[0096] Put 1g of syrup sample in a 50mL centrifuge tube, add 20mL of extractant (the extractant is mixed with equal volumes of methanol containing 1% formic acid and ultrapure water), vortex and mix until the syrup sample is completely dissolved, and place the centrifuge tube in an ultrasonic cle...

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Abstract

The invention discloses an analytical method for identifying Chinese date honey and syrup-adulterated with fake Chinese date honey, and particularly relates to a method by applying the ultra-high performance liquid chromatography-quadrupole rod-orbitrap high resolution mass spectrum technique combining metabonomics. The method comprises the steps that firstly, a true Chinese date honey sample and a syrup-adulterated with fake Chinese date honey sample to be detected are pretreated by organic solvent respectively, separation and determination of chemical components in the pretreated samples are achieved by applying an ultra-high performance liquid chromatography-quadrupole rod-orbitrap high resolution mass spectrometry method, then obtained UHPLC-MS original data of the true Chinese date honey sample and the Chinese date honey sample to be detected is preprocessed, and finally true Chinese date honey and syrup-adulterated with fake Chinese date honey are distinguished by applying principal component analysis of a multivariate statistical analysis method. According to the analytical method, after metabolin information of Chinese date honey and syrup-adulterated with fake Chinese date honey is obtained comprehensively, combined with multivariate statistical analysis, Chinese date honey and syrup-adulterated with fake Chinese date honey are analyzed comprehensively, and the detection of syrup-adulterated with fake Chinese date honey is completed.

Description

technical field [0001] The invention belongs to the technical field of food adulteration identification, and in particular relates to an analysis method for identifying jujube nectar and syrup adulterated jujube nectar using a metabolomics technology combined with UHPLC-Q Exactive. Background technique [0002] Honey is a natural sweet substance that bees collect nectar, secretions or honeydew from plants, mix with their own secretions, and fully brew. Honey has high nutritional value, but due to the high price and low output of honey, it makes Honey has become a main object of adulteration by illegal traders. In order to improve the production enthusiasm of beekeepers, protect the interests of consumers, support the fair competition of formal honey production enterprises, maintain the order of the honey market, and promote the healthy development of my country's honey industry, therefore It is of great significance and value to find the marker metabolites of real honey sample...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88G01N2030/8809
Inventor 耿越宿书芳江瑶王骏王凯利祝建华
Owner SHANDONG NORMAL UNIV
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