A kind of compound culture medium of Pleurotus primary strain and preparation method thereof
A culture medium and the technology of Pleurotus genus are applied in the field of the first-class strain compound medium of Pleurotus genus and the field of preparation thereof, which can solve the problems of short growth cycle, waste of Sargassum algae resources, and many aerial hyphae, etc., and achieve a short growth cycle. , Conducive to large-scale industrial production, the effect of strong mycelium
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[0033] The preparation method of the sage polysaccharide crude extract of the present invention can refer to the preparation method of the prior art, such as microwave extraction, ultrasonic extraction or ultrasonic enzymatic extraction, the sage polysaccharide crude extract in the present embodiment 1-4 The preparation method is obtained by referring to the optimal technical scheme in the process research of Li Qingtao et al. for extracting sargassum polysaccharides by microwave method. The specific process is: microwave time 8min, microwave power 540W, solid-liquid ratio 1:30, ultrasonic time 3min, the obtained crude The polysaccharide content is 26.7%.
[0034] Preparation of 1L plate medium A: Peel the potatoes, dig out the teeth, wash and weigh 150g, slice and boil with water until the potato slices are cooked and not rotten, filter with four layers of gauze to obtain the filtrate . Weigh peptone 1.5g, VB 6 0.015mg, potassium dihydrogen phosphate 2.0g, magnesium sulfat...
Embodiment 1
[0048] Inoculate 5 tubes of Pleurotus ostreatus strains stored in the refrigerator on the slant activated mother culture medium under aseptic conditions, and culture in a constant humidity incubator at 25° C. and a relative humidity of 50% for 5 days in shading. After 5 days, select the activated strain with the best growth status and inoculate it in the center of the PDA plate under aseptic conditions to ensure that the colony edge is continuous and the mycelium grows evenly, so that the growth of the mycelium used in the next punching inoculation is the same or similar. Five small plates (small Petri dishes) were inoculated for each strain. Humidity incubator 25 ℃, relative humidity 50%, shading culture 8d.
[0049] Select the plate with the best growth status after 8 days of cultivation, under sterile conditions, on the culture medium with mycelium growing at the same distance from the center of the plate, use a puncher to punch into uniform small pieces, directly 5 mm in s...
Embodiment 2
[0053] Inoculate 5 tubes of Pleurotus citrinopileatus strains stored in the refrigerator on the slant activated mother seed medium under aseptic adjustment, and inoculate 5 tubes in a constant humidity incubator at 25° C., with a relative humidity of 50%, and culture in shading for 5 days. After 5 days, select the activated strain with the best growth status and inoculate it in the center of the PDA plate under aseptic conditions to ensure that the colony edge is continuous and the mycelium grows evenly, so that the growth of the mycelium used in the next punching inoculation is the same or similar. Five small plates (small Petri dishes) were inoculated for each strain. Humidity incubator 25 ℃, relative humidity 50%, shading culture 8d.
[0054] Select the plate with the best growth status after 8 days of cultivation, under sterile conditions, on the culture medium with mycelium growing at the same distance from the center of the plate, use a puncher to punch into uniform smal...
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