System for culturing regenerated haploid plant from winter jujube anther and culture method thereof

Abstract

The invention relates to the technical field of agriculture, in particular to a system for culturing a regenerated haploid plant from winter jujube anther and a culture method thereof. The system comprises an anther culture medium, a primary callus culture medium and a secondary callus culture medium, wherein the anther culture medium consists of 1 / 2 of MS, 0.8-1.2mol / L of 2, 4-D, 18-22g / L of maltose and 3.2-3.7g / L of agar; the primary callus culture medium consists of MS, 0.8-1.2mg / L of TDZ, 18-22g / L of maltose and 3.2-3.7g / L of agar; the secondary callus culture medium consists of MS, 1.8-2.2mg / L of triacontanol, 18-22g / L of maltose and 3.2-3.7g / L of agar. The system is simple in composition and facilitates culture; through haploid breeding, jujube trees can also adopt seed propagation to substitute asexual propagation, and a pure material can be provided for genetic research on a jujube variety with a highly heterozygous genotype, so that the breeding period is shortened and the research on the genetic theory of the jujube trees is promoted.

Description

technical field [0001] The invention relates to the field of agricultural technology, in particular to a haploid plant system for cultivating and regenerating winter jujube anthers and a culturing method thereof. Background technique [0002] In 1953, Tuleck used Ginkgo pollen to induce the formation of haploid callus for the first time on artificial medium. Guha and Maheshwari (1964) successfully obtained haploid from Datura inoxia anther culture for the first time. Later, it was confirmed that the haploid pollen plants were obtained from the embryoid body, and a new way of using pollen to breed haploid was created. In the past 30 years, the technology of using anther culture to produce haploid plants has been widely used. To 10 families, 24 genera, 34 species of more than 250 kinds of plants. The research on cultured haploids of fruit tree anthers started relatively late, and success was achieved in citrus, apple, grape, strawberry, litchi, longan and other tree species f...

AI Technical Summary

Problems solved by technology

[0003] Chinese Patent Publication No. CN104041414B discloses a method for obtaining haploid plants by culturing pepper anthers, including selecting flower buds, disinfecting flower buds, anther inoculation, pretreatment, anther culture, and inducing callus to redifferentiate into haploid plants; the invention Microspores and anther walls were co-cultured with solid medium, low temperature, heat shock pretreatment, callus induction, differentiation medium components and culture conditions were adjusted, and the callus rate and callus differentiation ability were improved; this method was used to carry out Pepper anther culture, callus induction rate of more than 60%, emergence rate of more than 80%, solved the problem of low induction rate of pepper anther culture

This method can obtain a large amount of winter jujube anther callus at about 70D; but the method is cumbersome and its application is limited