Rapid detection apparatus and detection method for residual organic pesticides in agricultural products

A technology of organic pesticides and detection devices, which is applied in the direction of measuring devices, preparation of test samples, and material analysis through observation of the influence of chemical indicators, which can solve the problems of long test time, high cost, and large investment in instruments. Achieve the effects of simple structure, convenient storage and use, and low cost

Active Publication Date: 2017-07-21
通标标准技术服务(天津)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The traditional pesticide detection methods are gas phase and liquid chromatography. In the existing technology, A/D conversion technology and 410nm independent c

Method used

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  • Rapid detection apparatus and detection method for residual organic pesticides in agricultural products
  • Rapid detection apparatus and detection method for residual organic pesticides in agricultural products
  • Rapid detection apparatus and detection method for residual organic pesticides in agricultural products

Examples

Experimental program
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Embodiment 1

[0041] Such as figure 1 , 2As shown, the present invention provides a rapid detection device for organic pesticide residues in agricultural products, including: a detection disk 100, which is a circular disk with a certain depression in the middle relative to its surrounding; Annular retaining ring 102 is detachably arranged vertically on the circular opening groove structure formed in the middle part of the upper end surface of said detection disc; It is radially distributed on the upper end surface of the detection disc, and part of the upper end surface of the detection disc around the central reaction area is divided into alternately arranged reagent storage areas 104 and interval areas 105, wherein there are 3 reagent storage areas. One is an enzyme reagent storage area, a substrate storage area or a blank area and a buffer reagent storage area thereof; the carrier includes a plurality of reagent carriers 200, which include filter paper and cellulose membranes that are s...

Embodiment 2

[0051] A detection method using a rapid detection device for organic pesticide residues in agricultural products, including the following steps:

[0052] Step 1, prepare phosphate buffer;

[0053] Step 2, prepare the sample, weigh 2g of lettuce, chop it and put it into a beaker or extraction bottle, add phosphate buffer, wherein, the mass volume ratio of sample to phosphate buffer is 1g:4-6ml, shake for 3~ 5min, pour out the extract, let stand for 4min, and set aside;

[0054] Step 3: Take 4 rapid detection devices for organic pesticide residues, open the vacuum coating film on the detection plate, and take out the desiccant embedded in the annular retaining ring;

[0055] Step 4, drop 1-2ml of phosphate buffer solution into one end of the cellulose membrane of the reagent storage area coated with acetylcholinesterase of four rapid detection devices for organic pesticide residues successively, so that the phosphate buffer solution is released from the cellulose membrane The ...

Embodiment 3

[0061] A detection method using a rapid detection device for organic pesticide residues in agricultural products, including the following steps:

[0062] Step 1, prepare phosphate buffer;

[0063] Step 2. Prepare the sample, weigh the apple, chop it and put it into a beaker or extraction bottle, add phosphate buffer, wherein the mass volume ratio of the sample to the phosphate buffer is 1g:4-6ml, shake for 3-5min , pour out the extract, let stand for 4min, and set aside;

[0064] Step 3: Take 4 rapid detection devices for organic pesticide residues, open the vacuum coating film on the detection plate, and take out the desiccant embedded in the annular retaining ring;

[0065] Step 4, drop 1-2ml of phosphate buffer solution into one end of the cellulose membrane of the reagent storage area coated with acetylcholinesterase of four rapid detection devices for organic pesticide residues successively, so that the phosphate buffer solution is released from the cellulose membrane T...

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Abstract

The invention discloses a rapid detection apparatus for residual organic pesticides in agricultural products. The rapid detection apparatus comprises a detection disc which is a circular disc body with a recessed central part relative to the periphery part; a central reaction zone formed by vertically mounting a circular-ring-shaped retainer ring on the central part of the upper end surface of the detection disc; a plurality of straight partition plates dividing the periphery of the central reaction zone into radial alternately-distributed reagent storage zones and spacer zones; a carrier which comprises a plurality of reagent carriers and a blank carrier located in the central reaction zone; and a vacuum coating film which coats the detection disc and the carriers. The rapid detection apparatus provided by the invention is simple in structure, low in cost and convenient to store and use. The invention also discloses a detection method using the apparatus. The detection method can visually and rapidly detect whether an agricultural product contains residual organic phosphorus and carbamate pesticides via color changes of the blank carrier in the central reaction zone; and the detection apparatus is convenient and rapid to use and greatly improves detection efficiency.

Description

technical field [0001] The invention relates to the technical field of pesticide residue detection in agricultural products, in particular to a rapid detection method for organic pesticide residues in agricultural products. Background technique [0002] As we all know, China is a big agricultural country, as well as a big pesticide producer and pesticide user. Pesticides play a vital role in preventing and controlling pests, weeds, and regulating crop growth, thereby promoting stable and high yields of crops. Especially in the production process of agricultural products such as vegetables and fruits, organophosphorus and carbamate pesticides are widely used. These two types of pesticides have been used in agriculture for a long time and are highly toxic to humans and animals. With the improvement of people's living standards, the problem of pesticide residues in the environment and the residues of agricultural products with pesticide residues has received widespread attentio...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N1/28G01N1/34
CPCG01N1/286G01N1/34G01N21/78G01N2001/2873
Inventor 朱敏张健
Owner 通标标准技术服务(天津)有限公司
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