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A kind of monoterpene compound and its preparation method and application

A compound and monoterpenoid technology, applied in the field of monoterpenoids and their preparation, can solve the problems of less reports on pharmacological activity and the like

Inactive Publication Date: 2020-04-17
YUNNAN MINZU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are few reports on the pharmacological activities of Nepeta chinensis plants and their chemical constituents

Method used

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  • A kind of monoterpene compound and its preparation method and application
  • A kind of monoterpene compound and its preparation method and application
  • A kind of monoterpene compound and its preparation method and application

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preparation example Construction

[0026] The preparation method of the monoterpenoid compound according to the present invention is obtained by using Nepeta chinensis leaves as raw material, extracting from extract, extracting with organic solvent, silica gel column chromatography, Sephadex LH-20 column chromatography and high pressure liquid chromatography. ,Specifically:

[0027] A. Extraction of extract: dry and pulverize the Nepeta leaves to obtain particles with a particle size of 0.1-0.2 cm, add 85-100% ethanol solution with a volume percentage concentration of 5-10 times the weight of Nepeta leaves at a temperature of 64- Reflux extraction at 74°C for 3 to 5 times, each time for 1.5 to 3 hours, combine the extracts, filter, and concentrate the filtrate under reduced pressure to a specific gravity of 1.1 to 1.3 to obtain extract a;

[0028] B. Organic solvent extraction: Add 3 to 5 times the weight of extract a to extract a, and then use petroleum ether, ethyl acetate and n-butanol solvents equal to the ...

Embodiment 1

[0043] Source of material: Nepeta nepeta was collected in Kunming, Yunnan, and was identified as Chenopodium ambrosioides L., the specimen is preserved in the Herbarium of the College of Ethnic Medicine, Yunnan University for Nationalities.

[0044] a. Pulverizing: dry and pulverize 6Kg of Schizonepeta leaves into particles with a particle diameter of 0.10 cm to obtain Schizonepeta powder for subsequent use;

[0045] B. reflux extraction: the Nepeta nepeta powder prepared in step a was reflux extracted at a temperature of 65° C. for 4 times, each time for 2 hours, each time extracted with 30Kg 85% ethanol, combined with the ethanol extract, and set aside;

[0046] c. Concentration: filter the ethanol extract prepared in step b through 80-120 micron filter paper and concentrate under reduced pressure with a rotary evaporator at a temperature of 50°C until the specific gravity is 1.2 to obtain 580g of extract, which is set aside;

[0047] d, extraction: suspend 580g extract in...

Embodiment 2

[0050] a. Pulverization: dry and pulverize 6Kg of Schizonepeta leaves into particles with a particle diameter of 0.15 cm to obtain Schizonepeta powder for subsequent use;

[0051] B. reflux extraction: the Nepeta nepeta powder prepared in step a was refluxed and extracted 4 times at a temperature of 70° C., each time for 1.5 hours, extracted with 36Kg 88% ethanol each time, combined with the ethanol extract, and set aside;

[0052] c. Concentration: filter the ethanol extract prepared in step b through 80-120 micron filter paper and concentrate under reduced pressure with a rotary evaporator at a temperature of 50°C until the specific gravity is 1.1 to obtain 576g of extract, which is set aside;

[0053] d, extraction: suspend 576g extract in 2300ml water, extract successively with 2300ml sherwood oil, 2300ml ethyl acetate and 2300ml n-butanol, extract 5 times with each solvent, then concentrate under reduced pressure with a rotary evaporator to obtain respectively Petroleum e...

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Abstract

The invention discloses a monoterpene compound as well as a preparation method and application thereof. The monoterpene compound is obtained by taking chenopodium ambrosioides leaves as a raw material, extracting extract, extracting with an organic solvent, carrying out silica gelcolumn chromatography, carrying out Sephadex LH-20 column chromatography and carrying out high pressure liquid chromatography separation, wherein the molecular formula of the compound is C10H16O2, the compound is named 4-hydroxy-4-isopropyl-2-methyl-2-cyclohexene-1-one, and the compound has a structure (described in the specification). The preparation method comprises the following steps: by taking the chenopodium ambrosioides leaves as the raw material, extracting the extract, extracting with the organic solvent, carrying out the silica gelcolumn chromatography, carrying out the Sephadex LH-20 column chromatography and carrying out the high pressure liquid chromatography separation, so that the monoterpene compound is obtained. The application is namely application of the monoterpene compound in preparation of a drug used for preventing and / or inflammation. In vitro anti-inflammation activity tests are carried out on the monoterpene compound, and experimental results show that the monoterpene compound has good effect of inhibiting lipopolysaccharide (LPS)-induced mouse macrophage (RAW264.7) from producing nitric oxide (NO) and can provide a lead compound with application value for medical industry.

Description

technical field [0001] The invention belongs to the technical field of extraction of plant active ingredients, and in particular relates to a monoterpene compound and its preparation method and application. Background technique [0002] Inflammation is a kind of non-specific immune response mainly based on defense adaptation produced by the body to the stimulation and damage of various inflammatory factors. The pathogenesis of many diseases is related to inflammation, common clinical rheumatoid, arthritis, asthma, trauma, burns, septic shock, heart failure, etc. all have the participation of inflammatory mediators (cytokines). Anti-inflammatory drugs can block the production or release of inflammatory mediators, inhibit inflammatory reactions, and thus treat diseases related to inflammatory mediators. At present, the treatment of acute and chronic inflammation at home and abroad is mainly based on antibiotics. Although the treatment of these drugs can receive good results, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C45/78C07C45/79C07C49/713A61P29/00
CPCA61K36/185C07C45/78C07C45/79C07C49/713
Inventor 李艳红黄相中田凯杨淬孙静贤钏永明李蓉
Owner YUNNAN MINZU UNIV
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