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Rotary nucleic acid detection card case

A detection card and rotary technology, applied in the field of microbial detection, can solve the problems of complicated operation and long time-consuming, and achieve the effect of reducing infection, avoiding false positives, and speeding up the detection speed.

Pending Publication Date: 2017-08-11
HUNAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Food safety incidents are sudden and urgent, and the detection results of food contamination sources also need timeliness, while t

Method used

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  • Rotary nucleic acid detection card case
  • Rotary nucleic acid detection card case
  • Rotary nucleic acid detection card case

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Embodiment Construction

[0030] The invention relates to nucleic acid detection of microorganisms in samples. Microorganisms can be bacteria, viruses and fungi, among others. The reagent tank of the base is pre-loaded with binding solution, lysate, cleaning solution, eluent, mineral oil, LAMP reaction solution, and a detection chamber is included in the reagent chamber of the cartridge. For the detection of target DNA or RNA.

[0031] First, fill the pre-treated sample into the sample chamber inside the cartridge reagent chamber with a manual pipette, and then place the cartridge in the integrated detector as required. The detector is a matching card box equipment. Driven by external equipment, the internal pipetting component of the reaction box starts to process the sample liquid according to the preset program, and then goes through the process of binding solution, lysing solution, cleaning solution, etc., and then moves the extracted nucleic acid solution to the detection chamber, and finally M...

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Abstract

The invention discloses a card case used for sample pretreatment and nucleic acid detection. The card case comprises a pipettor, a vertical guide rod, a shell, a sealing cover, a base, at least two sealing rings and at least two springs. An accurately quantitative constant-speed and located rotatable liquid absorption/separation device is internally arranged; and at least one reagent chamber is formed in the base.

Description

technical field [0001] The invention belongs to the field of microorganism detection, in particular to the detection of on-site pathogenic microorganisms, and in particular to a rotary nucleic acid detection cartridge. The present invention combines magnetic bead nucleic acid extraction technology with nucleic acid detection technology (such as LAMP turbidity detection technology), and realizes automatic nucleic acid extraction / purification, amplification, and detection processes in a closed cartridge. Background technique [0002] The traditional microbial culture method is to add specific biochemical reaction substrates, antibodies, fluorescent reaction substrates, enzyme reaction substrates, etc. into the culture medium to select, separate and identify the detection objects. The operation process is cumbersome and time-consuming, and there is a risk of infection; different bacteria require different culture media, and most bacteria in a non-culturable state cannot be dete...

Claims

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Application Information

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IPC IPC(8): C12M1/34C12M1/00
CPCC12Q1/6844C12Q2531/119
Inventor 陈柱何农跃李松聂立波邓燕
Owner HUNAN UNIV OF TECH