Reagent plate for automatic pretreatment of nucleic acid detection

A reagent plate and nucleic acid sequencing technology, applied in the direction of biomass post-treatment, biomass pre-treatment, bioreactor/fermenter for specific purposes, etc., can solve the problems of unattended, sample impact, etc., to eliminate samples Effects of confusion, avoiding tediousness, and speeding up testing

Inactive Publication Date: 2017-02-15
HANGZHOU GENE META MEDICAL DEVICE CO LTD
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

Since the liquid pipetting workstation is an open platform, during the whole multi-step test operation, the operator needs to seal and open the 96-well plate film, or open and close a large number of tube caps from time to time, although part of the work is done by the workstation. Completed, but the overall still requires a lot of manual intervention, and it is impossible to realiz

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  • Reagent plate for automatic pretreatment of nucleic acid detection
  • Reagent plate for automatic pretreatment of nucleic acid detection
  • Reagent plate for automatic pretreatment of nucleic acid detection

Examples

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Example Embodiment

[0040] Example 1

[0041] like figure 1 The shown nucleic acid detection preprocessing automation device is composed of a moving rail platform 1 , a base 2 , a movable magnet 3 , a pipetting pump 4 and a pipetting tip 5 . The pipette tip 5 is arranged on the pipette pump 4, and the pipette pump can move along the moving rail platform 1 and rotate through the rotating module. The mobile rail platform 1 moves and rotates on the upper part of the reagent tray through the pipette pump and the pipette tip to realize pipetting. The base 2 has a temperature control module corresponding to the position of the reagent tray. Base 2 fits the reagent tray to provide temperature control. The reagent tray can be rotated and moved through the rotation module on the base 2 .

[0042] like Figure 2-3 The reagent tray shown includes a cover plate 6 and a reagent tray 7. The reagent tray 7 is provided with several reagent holes 8. The reagent holes 8 are filled with nucleic acid sequencing...

Example Embodiment

[0044] Example 2

[0045] Plasma cell-free DNA is small fragment and fragmented DNA, the fragment length is between 100 and 300 bp, and the peak value is about 160 bp. In 1997, Professor Lu Yuming discovered that fetal cell-free DNA was contained in the peripheral plasma of pregnant women, thus pioneering the use of cell-free DNA as the source of detection to analyze whether the fetus carried by pregnant women had chromosomal abnormalities. Circulating tumor DNA (ctDNA) derived from tumor cells also exists in plasma. ctDNA is widely used for tumor liquid biopsy, which can be used for non-invasive early diagnosis, companion diagnosis, and prognosis follow-up.

[0046] The conventional detection method is to first extract DNA from clinical samples including plasma, and then perform three steps of nucleic acid detection processing and detection. It takes 6 to 13 hours to complete the entire sample test. Among them, sample nucleic acid extraction and pre-detection processing are...

Example Embodiment

[0063] Example 3

[0064] The research object of transcriptome sequencing is the sum of all RNAs that can be transcribed by a specific cell in a functional state, mainly including mRNA and non-coding RNA. Transcriptome research is the basis and starting point of gene function and structure research. Through next-generation high-throughput sequencing, almost all transcript sequence information of a specific tissue or organ of a species can be obtained comprehensively and quickly in a certain state, which has been widely used. In basic research, clinical diagnosis and drug development and other fields.

[0065] The conventional detection method is to first extract RNA from clinical samples including whole blood and plasma, and carry out three steps of nucleic acid detection processing and detection. It takes 6 to 30 hours to complete the entire sample test. Among them, sample nucleic acid extraction and pre-detection processing are cumbersome.

[0066] The nucleic acid detect...

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Abstract

The invention discloses a reagent plate for automatic pretreatment of nucleic acid detection. The reagent plate is characterized by comprising a cover plate and a reagent tray, wherein a plurality of reagent holes are formed in the reagent tray; nucleic acid sequencing pretreatment reagents are contained in the reagent holes; aluminum foil sealing films are arranged at the opening of the reagent holes. The reagent plate for automatic pretreatment of nucleic acid detection, provided by the invention, in combination with an automatic pretreatment device for nucleic acid detection, can realize the full automatic pretreatment of the nucleic acid detection, including extraction, library construction and product purification; the final products can be directly applied to high-throughput sequencing; the whole process is unmanned.

Description

technical field [0001] The invention belongs to the field of nucleic acid molecular detection, and in particular relates to a reagent disk for automatic pretreatment of nucleic acid detection. The present invention realizes fully automatic nucleic acid detection pre-processing, that is, automatically realizes pre-processing such as nucleic acid extraction, amplification, and purification, and the processed samples can be detected by an external matching sequencer. The present invention simplifies the test process, reduces the chance of error, and improves The stability of the overall detection is improved. Background technique [0002] High-throughput sequencing technology is a new molecular detection technology developed in the last decade. The mainstream high-throughput sequencers on the market are mainly provided by Illumina, ThermoFisher, etc. Any high-throughput sequencing platform will first require DNA or RNA to be extracted from biological samples before testing, an...

Claims

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Application Information

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IPC IPC(8): C12M1/00
CPCC12Q1/6806C12Q2523/308C12Q2531/113C12Q2535/122
Inventor 叶宝春黄飞祝云英
Owner HANGZHOU GENE META MEDICAL DEVICE CO LTD
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