Method for establishing mutant strain by utilizing plasma induced lucid ganoderma protoplast
A protoplast and plasma technology, which is applied in the field of crop mutation breeding, can solve the problems of harvesting season limitation, difficult to achieve mutagenesis effect, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0041] A method of utilizing plasma mutagenesis ganoderma lucidum strain cgmcc 5.0026 protoplasts of the present embodiment to construct mutant strains comprises the following steps:
[0042] (1) Construction of Ganoderma lucidum protoplasts
[0043] ①Under sterile conditions, inoculate small pieces of mycelium of solid fermented Ganoderma lucidum seeds into sterilized PD liquid medium (1L sterile water, 200g potatoes, 20g glucose), and cultivate them on a shaking table at 27°C and 150rpm , after 5 days, the mycelium and the bacterial solution were taken out, centrifuged in an ultra-clean bench and washed with ddH 2 O cleaning mycelium;
[0044] ②Take 5mL Ganoderma lucidum mycelium and place it in a sterilized homogenizer, add an appropriate amount of mannitol at a concentration of 0.6mol / L; grind it in the homogenizer, and prepare mycelium homogenate in an ultra-clean bench; Observe that the length of the mycelia is appropriate, centrifuge at 2000rpm for 6 minutes to obtain...
Embodiment 2
[0061] A method of using plasma mutagenesis of Chibaihuang Ganoderma lucidum protoplasts to construct mutant strains in this embodiment comprises the following steps:
[0062] (1) Construction of Ganoderma lucidum protoplasts
[0063] ①Under sterile conditions, inoculate small pieces of solid fermented Ganoderma lucidum seed mycelium into sterilized PD liquid medium (1L sterile water, 200g potatoes, 20g glucose), and culture at 29°C and 200rpm on a shaking table After 7 days, the mycelium and the bacterial solution were taken out, centrifuged in the ultra-clean bench and washed with ddH 2 O cleaning mycelium;
[0064] ②Take 5mL Ganoderma lucidum mycelium and place it in a sterilized homogenizer, add an appropriate amount of mannitol at a concentration of 0.6mol / L; grind it in the homogenizer, and prepare mycelium homogenate in an ultra-clean bench; Observe that the length of the mycelia is appropriate, centrifuge at 5000rpm for 4 minutes to obtain a homogenate of the mycelia...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com