Method for permanently preserving externalia of small-size insects

A technology of external genitalia and preservation method, which is applied in the field of permanent preservation of small insect external genitalia, which can solve the problems of not being able to display the important shape and character characteristics of the structure from different angles, and the one-time slide cannot be checked repeatedly, so as to save storage space.

Pending Publication Date: 2017-09-15
GUIZHOU NORMAL UNIVERSITY
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  • Abstract
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Problems solved by technology

[0003] To sum up, the problems existing in the existing technology are: in the existing technology, important classified tissues such as the external genitalia of insects are

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  • Method for permanently preserving externalia of small-size insects

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Embodiment Construction

[0019] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0020] The application principle of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0021] Such as figure 1 As shown, the small insect external genitalia permanent preservation method provided by the embodiments of the present invention comprises the following steps:

[0022] S101: the collected fresh specimen (insect living state) is immediately placed in a test tube of absolute ethanol (100%) and brought back to the laboratory;

[0023] S102: Store in a low-temperature refrigerator at -35°C to -40°C;

[0024] S103: Take out the specimen from absolute ethanol, use...

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Abstract

The invention belongs to the technical field of insect specimen preservation, and discloses a method for permanently preserving externalia of small-size insects. The method for permanently preserving the externalia of the small-size insects comprises the following steps: putting acquired fresh specimens into test tubes with absolute ethyl alcohol, and bringing back to a laboratory; storing the test tubes in a low-temperature refrigerator of minus 35 DEG C to minus 40 DEG C; and identifying varieties, taking out the specimens, taking off externalia from abdomen, dissecting, observing different parts, bleaching for 3-4 times in absolute ethyl alcohol, diluting extra water or even removing extra water, and preserving in transparent glass devices with triglyceride. By adopting the method, the problem that dead insect specimens are very liable to rot and deteriorate is solved, the insect specimens can be well preserved for a long time or permanently, and moreover important structures can be repeatedly taken out and observed.

Description

technical field [0001] The invention belongs to the technical field of insect specimen preservation, and in particular relates to a method for permanent preservation of small insect external genitalia. Background technique [0002] At present, most people use moth balls to repel insects to protect insect specimens. This method needs to make all specimens and put them in specimen boxes, which is time-consuming and laborious, and takes up a lot of space. If all the specimens are made into dry specimens and stored, they will be easily eaten by booklice, etc., and at the same time, the integrity of the gene sequence in the body will be lost to a large extent, and they cannot be used for gene extraction in subsequent molecular experiments. The permanent preservation method not only allows insect specimens to be permanently stored, but also saves storage space, and can be directly used for the extraction of genes in molecular experiments. The existing technology is to use gum cana...

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Application Information

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IPC IPC(8): A01N1/00
CPCA01N1/00
Inventor 宋月华李灿高娅蓉熊康宁
Owner GUIZHOU NORMAL UNIVERSITY
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