A kind of Lactobacillus paracasei, its preparation and its application in pig feed

A technology of Lactobacillus and paracasei, which is applied in preparations and its application in pig feed, in the field of Lactobacillus paracasei, can solve the problems of long time spent in the fermentation process and cumbersome production operations, and reduce the number of viable bacteria and Harmful bacteria emission and diarrhea rate reduction effect

Active Publication Date: 2020-03-24
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Lactobacillus paracasei involved in the above patents is mainly used for the preparation of fermented feed. The preparation process requires fermentation equipment, the fermentation process takes a long time, and the production operation is relatively cumbersome. At present, there is no freeze-dried preparation involving Lactobacillus paracasei The preparation method also does not involve the method that the freeze-dried preparation with stable quality is directly added to the feed in proportion and can improve the reproductive performance of sows

Method used

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  • A kind of Lactobacillus paracasei, its preparation and its application in pig feed
  • A kind of Lactobacillus paracasei, its preparation and its application in pig feed
  • A kind of Lactobacillus paracasei, its preparation and its application in pig feed

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Experimental program
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Effect test

Embodiment 1

[0038] Isolation, screening and identification of embodiment 1 Lactobacillus paracasei

[0039] Select healthy nursery pigs to slaughter, take out the intestines by aseptic operation, scrape the intestinal mucosa, mix with sterile saline, and dilute ten times to 10 -5 、10 -6 、10 -7 , 0.1 mL each was spread on the MRS plate medium, and cultured at 37° C. for 24 to 48 hours by anaerobic jar method. Pick milky white colonies from them for preliminary screening, all Gram-stained positive, H 2 o 2 The strains with negative enzyme test and negative contact enzyme can be preliminarily identified as lactic acid bacteria. The strains obtained from the preliminary screening are analyzed for acid resistance, bile salt resistance, and bacteriostasis. Finally, a strain of lactic acid bacteria ZLP019 with excellent performance is screened out and sent to China General Hospital. The Microbial Culture Collection Center (CGMCC) identified the bacteria as Lactobacillus paracasei through compr...

Embodiment 2

[0045] The characteristic research of embodiment 2 Lactobacillus paracasei

[0046] 1. Growth curve of Lactobacillus paracasei

[0047] The Lactobacillus paracasei obtained by the activated embodiment 1 is inserted into the MRS liquid medium with an inoculum size of 1%, and an automatic microbial growth curve analyzer is used to detect the OD 600 Value, draw the growth curve, the result is as figure 2 , where the abscissa is the culture time in hours, and the ordinate is the absorbance OD 600 value. The experimental results showed that Lactobacillus paracasei grew rapidly in the MRS medium, entered the logarithmic phase at about 4h, and entered the stationary phase at about 11h.

[0048] 2. Determination of acid resistance

[0049] Insert the activated Lactobacillus paracasei into the MRS liquid medium with pH of 2, 3 and 4 respectively according to the inoculum amount of 1%, and incubate at 37°C for 2, 4 and 6h respectively, and use 0h as a control to measure the number ...

Embodiment 3

[0070] The preparation of embodiment 3 Lactobacillus paracasei preparation

[0071] 1. Fermentation and cultivation of Lactobacillus paracasei

[0072] Inoculate the Lactobacillus paracasei (Lactobacillus paracasei) ZLP019 (preservation number: CGMCC No.11532) obtained in Example 1 in the MRS liquid medium with a 1% inoculum size, and culture it at 37° C. for 16 to 24 hours, so as to activate 2 generations Obtain seed culture afterward; Seed culture is inoculated in the 50L fermenter that 30L liquid culture medium is housed with 1% inoculum size, set temperature 37 ℃, rotating speed 100rpm, keep constant pH 5.5, when fermenting to 14 hours Feed feeding was started, and 200 mL of feeding liquid was added to the fermenter at a time, and then feeding was carried out every 2 hours for a total of 5 feedings; when the pH of the fermentation broth no longer decreased, the fermentation was terminated.

[0073] Among them, the composition of the liquid medium is: glucose 30g / L, pepton...

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Abstract

The invention discloses a Lactobacillus paracasei (Lactobacillus paracasei) ZLP019, and the preservation number is: CGMCC No.11532. The bacteria has good acid resistance, bile salt resistance and antibacterial properties. The bacterium is used to prepare a freeze-dried preparation, the survival rate of live bacteria is high, and it is convenient to use in feed enterprises and farms. The preparation is directly added to the sow feed according to the proportion requirements, which can improve the reproductive performance of the sow, increase the daily weight gain and survival rate of the suckling piglet, promote the intestinal health of the sow, reduce the discharge of harmful bacteria in the feces, and benefit the breeding environment friendly.

Description

technical field [0001] The invention relates to the technical field of biological feed, in particular to a kind of Lactobacillus paracasei, its preparation and its application in pig feed. Background technique [0002] In pig production, sows undertake the important task of breeding piglets and suckling piglets. They have a long service life and play a big role. However, sows in large-scale pig farms often have problems such as constipation, decreased appetite, poor farrowing performance, low lactation, and low survival rate of piglets, which seriously affect the production performance and economic benefits of the farm. [0003] Lactic acid bacteria can regulate the intestinal microecological balance of animals, promote the digestion and absorption of nutrients, and promote the health of the body. Reasonable feeding is expected to improve the comprehensive production capacity of sows. [0004] Lactobacillus paracasei (Lactobacillus paracasei) is one of the species of Lactob...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/18A23K50/30C12R1/245
CPCC12N1/205C12R2001/245
Inventor 季海峰刘辉王四新张董燕王晶张伟王雅民
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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