Detection method for quickly screening multiple veterinary drug residues in fish flesh

A technology for veterinary drug residues and detection methods, which is applied in the field of food chemical analysis, can solve the problems of complex fish meat matrix and unsatisfactory detection limit, and achieve the effect of simple method, high sensitivity and good precision

Active Publication Date: 2017-09-15
INSPECTION AND QUARANTINE TECHNOLOGY CENTER ZHONGSHAN ENTRY EXIT INSPECTION AND QUARANTINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, due to the complex matrix of fish meat, which contains various components such as fat and protein, conventional processing methods, the detection limit of the target compound in Q-Exactive cannot meet the requirements of the relevant national health standards and announcements due to the interference of the matrix.

Method used

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  • Detection method for quickly screening multiple veterinary drug residues in fish flesh
  • Detection method for quickly screening multiple veterinary drug residues in fish flesh
  • Detection method for quickly screening multiple veterinary drug residues in fish flesh

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Embodiment

[0040] (1) Standard solution preparation: Accurately weigh oxacillin sodium, ampicillin, cloxacillin sodium, disodium penicillin sodium hydrate, ceftiofur, nafcillin sodium-hydrate, oxacillin sodium, phenoxy Methpenicillin potassium salt, piperacillin standard substance 5.0mg, in respective 5mL volumetric flasks, dissolve with acetonitrile aqueous solution (acetonitrile: water=30:70, v / v) and settle to the mark, make mass concentration 1mg / mL standard stock solution; accurately weigh 2-amino-5-benzimidazole, toltrazuril, clazuril, ketoprofen, meloxicam, acetaminophen, piroxicam, salicylic acid , sulindac, tolfenac, tolmetin sodium, 4-aminoantipyrine, 4-formylaminoantipyrine, 4-methylaminoantipyrine, phenylbutazone, 17α-hydroxypregnancy Ketone, melengestrol acetate, megestrol acetate, medroxyprogesterone, trendisolone, burgundone and nandrolone standard about 5.0mg, in their respective 5mL volumetric flasks, dissolved with acetonitrile and dilute to Make a standard stock solut...

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Abstract

The invention discloses a detection method for quickly screening multiple veterinary drug residues in fish flesh. The method comprises the following steps: preparing 31 kinds of standard solutions, determining the standard solutions, drawing a standard working curve, preprocessing a sample by adopting a QuEChERS technology to effective reduce the interference influences of matrixes on target compounds (including beta-lactam, non-steroidal anti-inflammatory drugs and other 29 kinds of veterinary drug residues), and determining the processed sample by a ultra-high performance liquid chromatograph-high resolution mass spectrometer. The method for determining the 31 kinds of veterinary drug residues in fish flesh has high sensitivity, and the detection limit is 0.1-2.5 [mu]g / kg; and the detection method also has the advantages of simplicity, rapidity, good accuracy, high sensitivity and good precision, is suitable for large-scale detection of fish flesh samples, can accurately quantitatively and qualitatively analyze the target compounds, and avoids the false positive phenomenon.

Description

【Technical field】 [0001] The invention relates to a detection method for quickly screening multiple veterinary drug residues in fish meat, and belongs to the technical field of food chemical analysis. 【Background technique】 [0002] With the intensive and large-scale rapid development of the modern breeding industry, in order to pursue greater commercial interests and reduce the risks brought about by high-density breeding, aquatic product farmers often abuse veterinary drugs. Another reason for veterinary drug residues is failure to comply with the drug withdrawal period and aquatic product hygiene regulations, causing veterinary drug residues in aquatic products. After these contaminated aquatic products enter the human body through the food chain, it is easy to cause accumulation of toxicity, bacterial drug resistance, etc. A series of chain hazards seriously threaten people's health. The use and residues of veterinary drugs in aquatic products have attracted more and mo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 张宪臣华洪波李蓉李浩洋卢俊文张朋杰容裕棠
Owner INSPECTION AND QUARANTINE TECHNOLOGY CENTER ZHONGSHAN ENTRY EXIT INSPECTION AND QUARANTINE
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