Gastric cancer molecular marker hsa_circ_0006633 and its application

A technology of molecular markers and gastric cancer, applied in the determination/testing of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of incomplete elucidation of the pathophysiological mechanism of gastric cancer, early diagnosis and further treatment limitations, etc. Achieve good gastric cancer tissue specificity, good sensitivity and specificity, and good gastric cancer correlation

Active Publication Date: 2017-09-19
叶国良
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  • Abstract
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  • Claims
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Problems solved by technology

[0004] In view of the occurrence and development of gastric cancer is a multi-factor, multi-stage, multi-step process, involving a large number of genes involved and complex network r

Method used

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  • Gastric cancer molecular marker hsa_circ_0006633 and its application
  • Gastric cancer molecular marker hsa_circ_0006633 and its application
  • Gastric cancer molecular marker hsa_circ_0006633 and its application

Examples

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Example Embodiment

[0030] Example 1

[0031] 1. Sample collection and processing

[0032] Perform surgical treatment on patients with gastric cancer and remove the diseased tissue. A piece of soy-sized tissue was cut from the gastric cancer lesion and 5cm away from the lesion respectively, and cut into small pieces of tissue no more than 0.5cm thick with a scalpel, and soaked in 1mL RNA fixer non-liquid nitrogen sample RNA preservation solution (Beijing Biotech) , Put it in -80℃ ultra-low temperature refrigerator for later use.

[0033] 2. Tissue RNA extraction

[0034] 1) Take the tissue specimen out of the ultra-low temperature refrigerator at -80℃, and thaw at room temperature. Take out the tissue block from the RNA fixer non-liquid nitrogen sample RNA storage solution, blot dry with filter paper, cut 50mg-100mg tissue at multiple points, place it in a 2mL RNase-free centrifuge tube, add 1mL Trizol reagent, and homogenize with a handheld After the device is fully homogenized to no visible solids, ...

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Abstract

The invention relates to a gastric cancer molecular marker hsa_circ_0006633 and its application. The nucleotide sequence of the molecular marker is shown as SEQ ID NO.1, and the molecular marker is a closed loop RNA molecular marker. The molecular marker has significant expression in a gastric cancer tissue and extremely good gastric cancer correlation. Through applying CircRNA chip, real-time fluorescence quantification PCR detection and other technologies and combining with characteristics of simplicity, economy and practicability, the feasibility of using circRNA as a gastric cancer screening marker is explored; the gastric cancer molecular marker is of significance in clinical diagnosis and application.

Description

technical field [0001] The invention belongs to the field of tumor markers and applications, in particular to a gastric cancer molecular marker hsa_circ_0006633 and applications thereof. Background technique [0002] Gastric cancer is one of the most common tumors in the world, accounting for 10.4% of the global deaths every year, ranking the third cause of cancer death. Gastric cancer has the characteristics of insidious onset, early misdiagnosis, easy metastasis and recurrence, etc., and my country is restricted by economic and medical conditions. The diagnosis rate of early gastric cancer is less than 10%. Patients are often in the advanced stage of gastric cancer when they are diagnosed. The 5-year survival rate is about 4-27%. At present, gastroscopic biopsy and its pathological results are the gold standard for the diagnosis of gastric cancer, but due to the high cost and invasiveness of the operation, it is greatly limited in the screening process of early gastric can...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6886C12Q2600/118C12Q2600/158
Inventor 叶国良陆蓉丹郭俊明陆佳敏邵永富
Owner 叶国良
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