Method for detecting environmental low-dose ionizing radiation

A technology for ionizing radiation and detection environment, applied in the field of radiation detection to achieve good sensitivity and specificity

Active Publication Date: 2021-11-09
ACADEMY OF MILITARY MEDICAL SCI
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no specific biological detection

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting environmental low-dose ionizing radiation
  • Method for detecting environmental low-dose ionizing radiation
  • Method for detecting environmental low-dose ionizing radiation

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0042] Example 1

[0043] 1. Cell culture: The cell lines used in the present invention lymphoblasts AHH-1 and 16HBE human bronchial epithelial cells, cells were cultured in 1640 medium containing 10% fetal calf serum and DMEM medium, the set 37 ℃, 5 % CO 2 Incubator subcultured.

[0044] 2. ray irradiation using a cobalt 60γ: AHH-1 cells were subcultured and 16HBE cells administered 60γ cobalt-ray irradiation. Wherein said cobalt 60γ ray radiation irradiation conditions 20 ~ 25 ℃, the irradiation distance is 3 m, the dose rate 2.81cGy / min. Charge control group (group not irradiated) cells (labeled 0Gy), washed with phosphate buffer and 0.5 hours after irradiation charged, 1 hour, 2 hours, 4 hours, 8 hours, 24 hours and 48 hours, cells ( labeled 0.5h, 1h, 2h, 4h, 8h, 24h and 48h). Radiation dosage 0.01Gy, 0.02Gy, 0.05Gy, 0.1Gy, 0.2Gy.

[0045] 3.AHH1 extraction of nuclear proteins and 16HBE

[0046] Extract nuclear proteins, the following steps:

[0047] 1) charged 1mLAHH-1 cell...

Example Embodiment

[0088] Example 2

[0089] 1. Take 16 women and 25 men's peripheral blood (3 ml) to extract lymphocytes for detection of this bottom value of the negative control group.

[0090] The present invention is based on the step of extracting lymphocytes in human peripheral blood lymphocyte isolation (Tianjin Ocean); this specification requires extracting lymphocytes at normal temperature, but the lymphocytes extracted at normal temperature are detected by the method of the present invention. The display has no signal, that is, γh2ax is detected. However, at 4 ° C extraction to lymphocytes for testing, weaker signals can be seen. Therefore, the invention is selected at 4 ° C when extracting lymphocytes. At the same time, since the speed setting in the human peripheral blood lymphocyte isolation is based on the solution designed under normal temperature conditions, the present invention selects the following centrifugal conditions to centrifugation: 500 g, 30 min centrifugation; 500g, 20 m...

Example Embodiment

[0103] Example 3

[0104] Construction of fit curves in human peripheral blood lymphocyte levels. That is, the value of γH2ax / h2ax (%) of γH2ax / h2ax (%) after 0.5 h, 0.5 h, 0.5 h, 0.5 h, radiation dose (0.01 gy, 0.02 Gy, 0.05 gy, and 0.02 gy, 0.01 gy, 0.1GY and 0.2GY) are constructed for horizontal coordinates, Figure 10 Indicated. The results show: the fitting equation is y = -104.99X 2 + 37.689x + 3,0409, R 2 = 0.9632, the degree of fit between the estimation value of the fitting curve and the corresponding actual data can be used to evaluate the radiation dose.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for detecting environmental low-dose ionizing radiation, and belongs to the technical field of radiation detection. The method comprises the following steps: 1) taking cells to be detected and cells of a negative control group, and respectively extracting cell nucleuses to obtain the cell nucleuses; 2) extracting histone from the cell nucleus obtained in the step 1) to obtain histone; (3) detecting the molar content of gamma H2AX and H2AX in the histone; 4) when the molar content ratio of gamma H2AX to H2AX of the cells to be detected is higher than that of the negative control group under the same treatment condition, judging that the cells are subjected to low-dose ionizing radiation; according to the fitted curve, obtaining the specific dose of the ionizing radiation on the cells to be detected by combining the molar content ratio of the gamma H2AX to the H2AX of the cells to be detected. According to the method, low-dose ionizing radiation in the environment can be detected, whether the ionizing radiation exists in the environment or not can be identified, and the radiation reference dose can be provided.

Description

technical field [0001] The invention relates to the technical field of radiation detection, in particular to a method for detecting low-dose ionizing radiation in the environment. Background technique [0002] Yangjiang and Enping in Guangdong, China are areas with high natural background radiation. Hospital radiologists, workers working in nuclear power plants, and patients who need to receive X-rays, CT and other radioactive medical examinations due to illness, and those who have long-term work in places with low-dose radiation Officers and soldiers in the environment may also have been exposed to low-dose radiation environments. Whether there is radiation in these environments and whether the accumulated dose has biological effects has not been confirmed by clear scientific research. [0003] Therefore, how to quickly and accurately identify and detect the radiation dose after the organism is exposed to the low dose of the environment has practical application value. At ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01T1/02G01N30/02G01N30/72
CPCG01T1/02G01N30/02G01N30/72G01N2030/027
Inventor 关华徐华周平坤谢剑炜赵红玲瞿敏敏谢达菲白琛俊
Owner ACADEMY OF MILITARY MEDICAL SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap