Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for detecting environmental low-dose ionizing radiation

A technology for ionizing radiation and detection environment, applied in the field of radiation detection to achieve good sensitivity and specificity

Active Publication Date: 2021-11-09
ACADEMY OF MILITARY MEDICAL SCI
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no specific biological detection method for environmental low-dose radiation in China

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting environmental low-dose ionizing radiation
  • Method for detecting environmental low-dose ionizing radiation
  • Method for detecting environmental low-dose ionizing radiation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] 1. Cell culture: The cell lines human lymphoblastoid AHH-1 and human bronchial epithelial cell 16HBE used in the present invention are cultured in 1640 medium and DMEM medium containing 10% fetal bovine serum respectively, and placed at 37° C. for 5 %CO 2 Subculture in an incubator.

[0044] 2. Irradiation with cobalt-60γ-rays: Subcultured AHH-1 cells and 16HBE cells were irradiated with cobalt-60γ-rays. Wherein, the irradiation conditions of cobalt-60γ-ray irradiation are 20-25° C., the irradiation distance is 3 meters, and the dose rate is 2.81 cGy / min. The cells (marked as 0Gy) of the control group (non-irradiated group) were collected, washed with phosphate buffered saline in turn, and the cells of 0.5 hour, 1 hour, 2 hours, 4 hours, 8 hours, 24 hours and 48 hours after irradiation were collected ( Marked as 0.5h, 1h, 2h, 4h, 8h, 24h and 48h). The irradiation doses were 0.01Gy, 0.02Gy, 0.05Gy, 0.1Gy, 0.2Gy, respectively.

[0045] 3. Extraction of nuclear protein...

Embodiment 2

[0089] 1. The peripheral blood (3mL) of 16 females and 25 males was extracted to extract lymphocytes for the detection of the background value of the negative control group.

[0090] The present invention is based on the steps of extracting lymphocytes from human peripheral blood lymphocyte separation fluid (Tianjin Hanyang); this specification requires extracting lymphocytes at room temperature, but the lymphocytes extracted at room temperature in the present invention are detected by the method of the present invention , showing no signal, that is, no γH2AX can be detected. However, when the lymphocytes were extracted at 4°C for detection, a weaker signal could be seen. Therefore, the present invention chooses to extract lymphocytes at 4°C. At the same time, because the rotational speed setting in the manual of human peripheral blood lymphocyte separation fluid is designed under normal temperature conditions, it is not suitable for extraction at low temperature. The present...

Embodiment 3

[0104] The fitting curve was constructed on the level of human peripheral blood lymphocytes. That is, the value of γH2AX / H2AX (%) after 0.01Gy, 0.02Gy, 0.05Gy, 0.1Gy and 0.2Gy low-dose irradiation of human peripheral blood for 0.5h is the ordinate, and the radiation dose (0.01Gy, 0.02Gy, 0.05Gy, 0.1Gy and 0.2Gy) are constructed as the abscissa, the result is as follows Figure 10 shown. The result shows: the fitting equation is y=-104.99x 2 +37.689x+3,0409,R 2 =0.9632, indicating that the fitting degree between the estimated value of the fitting curve and the corresponding actual data is high, which can be used to evaluate the received radiation dose.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for detecting environmental low-dose ionizing radiation, and belongs to the technical field of radiation detection. The method comprises the following steps: 1) taking cells to be detected and cells of a negative control group, and respectively extracting cell nucleuses to obtain the cell nucleuses; 2) extracting histone from the cell nucleus obtained in the step 1) to obtain histone; (3) detecting the molar content of gamma H2AX and H2AX in the histone; 4) when the molar content ratio of gamma H2AX to H2AX of the cells to be detected is higher than that of the negative control group under the same treatment condition, judging that the cells are subjected to low-dose ionizing radiation; according to the fitted curve, obtaining the specific dose of the ionizing radiation on the cells to be detected by combining the molar content ratio of the gamma H2AX to the H2AX of the cells to be detected. According to the method, low-dose ionizing radiation in the environment can be detected, whether the ionizing radiation exists in the environment or not can be identified, and the radiation reference dose can be provided.

Description

technical field [0001] The invention relates to the technical field of radiation detection, in particular to a method for detecting low-dose ionizing radiation in the environment. Background technique [0002] Yangjiang and Enping in Guangdong, China are areas with high natural background radiation. Hospital radiologists, workers working in nuclear power plants, and patients who need to receive X-rays, CT and other radioactive medical examinations due to illness, and those who have long-term work in places with low-dose radiation Officers and soldiers in the environment may also have been exposed to low-dose radiation environments. Whether there is radiation in these environments and whether the accumulated dose has biological effects has not been confirmed by clear scientific research. [0003] Therefore, how to quickly and accurately identify and detect the radiation dose after the organism is exposed to the low dose of the environment has practical application value. At ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01T1/02G01N30/02G01N30/72
CPCG01T1/02G01N30/02G01N30/72G01N2030/027
Inventor 关华徐华周平坤谢剑炜赵红玲瞿敏敏谢达菲白琛俊
Owner ACADEMY OF MILITARY MEDICAL SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com