A kind of soil dna extraction method

Abstract

The invention discloses a method for extracting DNA in soil. The method comprises the following steps: lysing a soil sample so as to obtain lysate by adopting an SDS (Sodium Sulfate)-lysozyme method; uniformly mixing the obtained lysate and 5*SDS Loading Buffera according to a ratio, adding the mixture into the obtained albumin glue comb holes; performing electrophoresis according to the conventional method, slightly taking out the albumin glue comb for staining after electrophoresis is ended; cutting off a silver target strip after the silver target strip appears, putting the target strip into a dialysis bag filled with 2 mL of PBS (Phosphate Buffer Solution), placing in a horizontal electrophoresis tank filled with a proper amount of 1*Tris-Gly electrophoretic buffer solution, and placing the horizontal electrophoresis tank on ice for performing electrophoresis; and placing the obtained dialysis bag in a small beaker filled with sterilized PBS, dialyzing at the temperature of 4 DEG C overnight, and collecting the supernatant in the dialysis bag on the next day, thereby obtaining the soil DNA extract. According to the method disclosed by the invention, the operating steps are simple, the DNA lost in the operation is less, and the obtained DNA amount is stable.

Description

technical field [0001] The invention relates to the field of soil DNA extraction, in particular to a soil DNA extraction method. Background technique [0002] At present, there are three main methods for soil total DNA extraction, direct method, indirect method and kit method. The direct method is to directly lyse the cells from the soil, but the current traditional method has too many steps, so the DNA yield is low, and the final DNA amount is unstable. The indirect method is to separate the cells first and then lyse, but many microorganisms are tightly bound to the soil particles, and the cells are not easy to separate, resulting in a low final DNA yield and a long time-consuming. The kit method has the advantages of being simple and fast, but it is expensive and uneconomical for the extraction of a large number of samples, and sometimes the DNA cannot be extracted. Contents of the invention [0003] In order to solve the above technical problems, the present invention...

AI Technical Summary

Problems solved by technology

The direct method is to directly lyse the cells from the soil, but the current traditional method has too many steps, so the DNA yield is low, and the final DNA amount is unstable

The indirect method is to separate the cells first and then lyse, but many microorganisms are tightly bound to the soil particles, and the cells are not easy to separate, resulting in a low final DNA yield and a long time-consuming

The kit method has the advantages of simplicity and speed, but it is expensive and uneconomical for the extraction of large quantities of samples, and sometimes the DNA cannot be extracted