Peanut leaf organization grinding system for extracting DNA

Abstract

The invention discloses a peanut leaf organization grinding system for extracting DNA, and belongs to the technical field of biotechnology. The peanut leaf organization grinding system comprises an alcohol lamp, a centrifugal tube, a liquid relief gun head and a liquid nitrogen, wherein a small end of the liquid relief gun head is provided with a circular ball after being heated and melted by the alcohol lamp; the centrifugal tube comprises a columnar section and a conical section, and the minimum diameter of the conical section is more than the diameter of the circular ball; the length of centrifugal tube is less than that of the liquid relief gun head; the circular ball formed by heating and melting the small end of the liquid relief gun head by the alcohol lamp is matched with the existing centrifugal tube; the peanut leaf organization after being cooled by the liquid nitrogen is smashed so as to directly extract DNA in the peanut leaf organization after smashing in the centrifugal tube; compared with the smashing of the peanut leaf organization realized by the mutual cooperation of a mortar and a bowl in the prior art, the waste of the peanut leaf organization during the grinding process is lightened; the peanut leaf organization grinding system is more suitable for extracting the peanut leaf organization DNA when the peanut leaf organization is few.

Description

technical field [0001] The invention relates to the technical field of biotechnology, in particular to a peanut leaf tissue grinding system for extracting DNA. Background technique [0002] Peanuts, also known as groundnuts, are annual herbaceous plants, known as "vegetable meat" because of their high oil content. In addition to being used for food, peanuts can also be used in printing and dyeing, papermaking industries, etc., and are important oil crops and economic crops in my country. my country's peanut planting area and total production rank among the top in the world, and it has strong international competitiveness. However, my country's current peanut germplasm resources are relatively limited, and some germplasm resources that are resistant to disease and stress are relatively scarce, especially those resistant to Aspergillus flavus. [0003] Although conventional hybrid breeding and mutation breeding can improve the genetics of peanuts to a certain extent, it is d...

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Problems solved by technology

[0005] Because what adopt in the prior art is that the mortar and the pestle cooperate with each other to realize the grinding of the peanut leaf tissue, a large amount of groundnut leaf tissue grinding liquid adheres to the mortar and the pestle, resulting in waste of the peanut leaf tissue liquid. When few, more can't adopt the method in the prior art to carry out the extraction of peanut leaf DNA

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