Uses of artemether in preparation of preparations promoting steatolysis and improving carbohydrate metabolism
A fat decomposition and artemether technology, applied in the field of biomedicine, can solve problems such as the application potential of artemether that has not yet been discovered, and achieve the effects of improving diabetes-related symptoms and reducing body weight
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Embodiment 1
[0077] Example 1. Artemether induces browning of 3T3-L1 white preadipocytes during differentiation
[0078] 1.1 Materials
[0079] 1.1.1 3T3-L1 white adipose precursor cells
[0080] 1.1.2 DMEM medium (Gibco)
[0081] 1.1.3 Fetal bovine serum (Gibco)
[0082] 1.1.4 Calf serum (TBD)
[0083] 1.1.5 Biotin (Sigma)
[0084] 1.1.6 Penicillin and streptomycin
[0085] 1.1.7 Artemether (Chengdu Pufeide Biotechnology Co., Ltd.)
[0086] 1.1.8 Trizol (Life Technology)
[0087] 1.1.9 Reverse Transcription Kit (ThermoScientific)
[0088] 1.1.10 Power SYBR Green Master Mix (Thermo Scientific)
[0089] 1.2 Equipment
[0090] 1.2.1 Thermo Scientific Forma Series II Water Jacket
[0091] 1.2.2 Applied Biotech 7500real time PCR System
[0092] 1.2.3 Microscope Olympus IX711
[0093] 1.2.5 Eppendorf Centrifuge 5427R
[0094] 1.2.6 Nucleic acid quantitative instrument Colibri
[0095] 1.3 Solution
[0096] 1.3.1 Medium
[0097] 3T3-L1 maintenance medium
[0098]
[0099] 3T3...
Embodiment 2
[0135] Example 2 Artemether induced brown fat-like characteristics of C3H10T1 / 2 mesenchymal stem cells in the process of differentiation into adipocytes
[0136] 2.1 Materials
[0137] 2.1.1 C3H10T1 / 2 mesenchymal stem cell line
[0138] 2.1.2 DMEM medium (Gibco)
[0139] 2.1.3 Fetal bovine serum (Gibco)
[0140] 2.1.4 Calf serum (TBD)
[0141] 2.1.5 Biotin (Sigma)
[0142] 2.1.6 Penicillin and streptomycin
[0143] 2.1.7 Artemether (Chengdu Pufeide Biotechnology Co., Ltd.)
[0144] 2.1.8 BMP4 (R&D)
[0145] 2.1.9 BMP7 (Prospec)
[0146] 2.1.8 PVDF membrane (Millipore Immunobilon)
[0147] 2.1.9 Chemiluminescence liquid numECL ultra (New Saimei Biotechnology Co., Ltd.)
[0148] 2.1.10 MitoTracker Green (Life Technology)
[0149] 2.1.11 Other unmentioned reagents were purchased from Sigma
[0150] 2.2 Equipment
[0151] 2.2.1 Thermo Scientific Forma Series II Water Jacket
[0152] 2.2.2 Microscope Olympus IX711
[0153] 2.2.3 Electrophoresis instrument BioRad Power...
Embodiment 3
[0189] Example 3 Artemether makes the vascular stromal fraction (Stromal Vascular Fraction, SVF) obtain brown adipocyte-like characteristics during the differentiation process
[0190] 3.1 Materials
[0191] 2.1.1 Male C57BL / 6J mice (4-6week, Shanghai Slack Experimental Animal Co., Ltd.)
[0192] 2.1.2 DMEM / F12 (1:1) medium (Gibco)
[0193] 2.1.3 Fetal bovine serum (Gibco)
[0194] 2.1.4 Biotin (Sigma)
[0195] 2.1.5 Penicillin and streptomycin
[0196] 2.1.6 Artemether (Chengdu Pufeide Biotechnology Co., Ltd.)
[0197] 2.1.7 PVDF membrane (Millipore Immunobilon)
[0198] 2.1.8 Chemiluminescence reagent numECL ultra (Xin Saimei Biotechnology Co., Ltd.)
[0199] 2.1.10 Other unmentioned reagents were purchased from Sigma
[0200] 3.2 Equipment
[0201] 3.2.1 Thermo Scientific Forma Series II Water Jacket
[0202] 3.2.2 Microscope Olympus IX711
[0203] 3.2.3 Electrophoresis instrument BioRad PowerPac HC
[0204] 3.2.4 Automatic Chemiluminescence Instrument ImageQuant...
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