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Uses of artemether in preparation of preparations promoting steatolysis and improving carbohydrate metabolism

A fat decomposition and artemether technology, applied in the field of biomedicine, can solve problems such as the application potential of artemether that has not yet been discovered, and achieve the effects of improving diabetes-related symptoms and reducing body weight

Inactive Publication Date: 2017-11-17
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Potential of artemether for weight control and metabolic improvement has not yet been found

Method used

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  • Uses of artemether in preparation of preparations promoting steatolysis and improving carbohydrate metabolism
  • Uses of artemether in preparation of preparations promoting steatolysis and improving carbohydrate metabolism
  • Uses of artemether in preparation of preparations promoting steatolysis and improving carbohydrate metabolism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Example 1. Artemether induces browning of 3T3-L1 white preadipocytes during differentiation

[0078] 1.1 Materials

[0079] 1.1.1 3T3-L1 white adipose precursor cells

[0080] 1.1.2 DMEM medium (Gibco)

[0081] 1.1.3 Fetal bovine serum (Gibco)

[0082] 1.1.4 Calf serum (TBD)

[0083] 1.1.5 Biotin (Sigma)

[0084] 1.1.6 Penicillin and streptomycin

[0085] 1.1.7 Artemether (Chengdu Pufeide Biotechnology Co., Ltd.)

[0086] 1.1.8 Trizol (Life Technology)

[0087] 1.1.9 Reverse Transcription Kit (ThermoScientific)

[0088] 1.1.10 Power SYBR Green Master Mix (Thermo Scientific)

[0089] 1.2 Equipment

[0090] 1.2.1 Thermo Scientific Forma Series II Water Jacket

[0091] 1.2.2 Applied Biotech 7500real time PCR System

[0092] 1.2.3 Microscope Olympus IX711

[0093] 1.2.5 Eppendorf Centrifuge 5427R

[0094] 1.2.6 Nucleic acid quantitative instrument Colibri

[0095] 1.3 Solution

[0096] 1.3.1 Medium

[0097] 3T3-L1 maintenance medium

[0098]

[0099] 3T3...

Embodiment 2

[0135] Example 2 Artemether induced brown fat-like characteristics of C3H10T1 / 2 mesenchymal stem cells in the process of differentiation into adipocytes

[0136] 2.1 Materials

[0137] 2.1.1 C3H10T1 / 2 mesenchymal stem cell line

[0138] 2.1.2 DMEM medium (Gibco)

[0139] 2.1.3 Fetal bovine serum (Gibco)

[0140] 2.1.4 Calf serum (TBD)

[0141] 2.1.5 Biotin (Sigma)

[0142] 2.1.6 Penicillin and streptomycin

[0143] 2.1.7 Artemether (Chengdu Pufeide Biotechnology Co., Ltd.)

[0144] 2.1.8 BMP4 (R&D)

[0145] 2.1.9 BMP7 (Prospec)

[0146] 2.1.8 PVDF membrane (Millipore Immunobilon)

[0147] 2.1.9 Chemiluminescence liquid numECL ultra (New Saimei Biotechnology Co., Ltd.)

[0148] 2.1.10 MitoTracker Green (Life Technology)

[0149] 2.1.11 Other unmentioned reagents were purchased from Sigma

[0150] 2.2 Equipment

[0151] 2.2.1 Thermo Scientific Forma Series II Water Jacket

[0152] 2.2.2 Microscope Olympus IX711

[0153] 2.2.3 Electrophoresis instrument BioRad Power...

Embodiment 3

[0189] Example 3 Artemether makes the vascular stromal fraction (Stromal Vascular Fraction, SVF) obtain brown adipocyte-like characteristics during the differentiation process

[0190] 3.1 Materials

[0191] 2.1.1 Male C57BL / 6J mice (4-6week, Shanghai Slack Experimental Animal Co., Ltd.)

[0192] 2.1.2 DMEM / F12 (1:1) medium (Gibco)

[0193] 2.1.3 Fetal bovine serum (Gibco)

[0194] 2.1.4 Biotin (Sigma)

[0195] 2.1.5 Penicillin and streptomycin

[0196] 2.1.6 Artemether (Chengdu Pufeide Biotechnology Co., Ltd.)

[0197] 2.1.7 PVDF membrane (Millipore Immunobilon)

[0198] 2.1.8 Chemiluminescence reagent numECL ultra (Xin Saimei Biotechnology Co., Ltd.)

[0199] 2.1.10 Other unmentioned reagents were purchased from Sigma

[0200] 3.2 Equipment

[0201] 3.2.1 Thermo Scientific Forma Series II Water Jacket

[0202] 3.2.2 Microscope Olympus IX711

[0203] 3.2.3 Electrophoresis instrument BioRad PowerPac HC

[0204] 3.2.4 Automatic Chemiluminescence Instrument ImageQuant...

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Abstract

The invention belongs to the field of biological medicines, relates to novel uses of artemether and artemisinin analogues in pharmacy, particularly relates to uses of artemether in preparation of preparations promoting steatolysis and improving carbohydrate metabolism, and more particularly relates to uses of artemether and artemisinin analogues in preparation of preparations promoting browning of white adipose and preparations reducing body weight and improving metabolism. In-vivo and in-vitro experiments prove that the artemisinin analogues have a function of inducing browning of white adipose, and through promoting the browning of white adipose, effects of reducing body weight, improving metabolism, maintaining a carbohydrate metabolism steady state, relieving fatty liver and improving diabetes related symptoms are achieved. Clinical application practices show that intolerable adverse effects of the artemisinin analogues have not been found, the artemisinin analogues can be further prepared into preparations promoting steatolysis and improving carbohydrate metabolism and the preparations are used for treating and improving metabolism related diseases.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to the new application of artemether and artemisinin analogues in pharmacy, in particular to the application of artemether in the preparation of preparations for promoting fat decomposition and improving glucose metabolism. In particular, it relates to the use of artemether and artemisinin analogues in the preparation of promoting the browning of white fat and in the preparation of preparations for reducing body weight and improving metabolism. Background technique [0002] Data show that obesity and the metabolic diseases it causes have developed into a global epidemic. According to the data released by the World Health Organization (WHO) in 2015, the obesity rate of the global population is as high as 13%; while in China, the adult obesity rate is 6.9%. Worryingly, obesity rates are higher among adolescents and children, and are increasing rapidly, which means that obesity rates will cont...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/357A61K31/366A61K31/541A61P3/04A61P3/10A61P3/06A61P1/16A61P5/50
CPCA61K31/357A61K31/366A61K31/541A61P3/04
Inventor 汤其群党永军路平李希张付闯左建平
Owner FUDAN UNIV
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