Novel application of massoia lactone and 5-hydroxy-2-caproleic acid

A technology of massoia lactone and decenoic acid, which is applied in the field of medicine, can solve the problems of few reports on pharmacological and pharmacological effects, and achieve the effect of reducing liver collagen fiber deposition, reducing light proline content, and good therapeutic effect

Active Publication Date: 2017-11-17
SHUGUANG HOSPITAL AFFILIATED WITH SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

5-Hydroxy-2-decenoic acid is the product after the hydrolysis of the lactone ring of massoia lactone (chemical structure see figure 1 ), which are only reported in the synthesis process of some compounds at present, and there are few reports on relevant pharmacological and pharmacodynamic studies
[0008] However, there are no reports of massoia lactone and 5-hydroxy-2-decenoic acid in the treatment of liver diseases

Method used

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  • Novel application of massoia lactone and 5-hydroxy-2-caproleic acid
  • Novel application of massoia lactone and 5-hydroxy-2-caproleic acid
  • Novel application of massoia lactone and 5-hydroxy-2-caproleic acid

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1 Experiment purpose

[0040] Study on the anti-fibrosis effect of massoia lactone and 5-hydroxy-2-decenoic acid

[0041] 2 Experimental methods

[0042] Experimental animals: The experimental animals used in the present invention are C57BL / 6 mice, weighing 20-22 g, male, provided by the Experimental Animal Center of Shanghai University of Traditional Chinese Medicine.

[0043]Carbon tetrachloride (CCL4) and olive oil used in the experiment were purchased from Sinopharm Chemical Reagent Co., Ltd.; massoia lactone was isolated from fermented Cordyceps mycelium powder in the laboratory, and 5-hydroxy-2-decenoic acid was The massoia lactone is hydrolyzed and converted by base and prepared by column chromatography, the structure is determined by NMR and MS, and the purity is greater than 98% by HPLC area normalization method.

[0044] Forty C57BL mice, SPF grade, were divided into normal group (Control, n=10), liver fibrosis model group (CCL4, n=10), massoia lactone group...

Embodiment 2

[0070] 1 Experiment purpose

[0071] Investigation of the Anticirrhosis Effects of Massoia Lactone and 5-Hydroxy-2-Decenoic Acid

[0072] 2 Experimental methods

[0073] Experimental animals: The experimental animals used in the present invention are C57BL / 6 mice, weighing 20-22 g, male, provided by the Experimental Animal Center of Shanghai University of Traditional Chinese Medicine.

[0074] Carbon tetrachloride (CCL4) and olive oil used in the experiment were purchased from Sinopharm Chemical Reagent Co., Ltd.; massoia lactone was isolated from fermented Cordyceps mycelium powder in the laboratory, and 5-hydroxy-2-decenoic acid was The massoia lactone is hydrolyzed and converted by base and prepared by column chromatography, the structure is determined by NMR and MS, and the purity is greater than 98% by HPLC area normalization method.

[0075] Forty C57BL mice, SPF grade, were divided into normal group (Control, n=10), liver cirrhosis model group (CCL4, n=10), massoia la...

Embodiment 3

[0101] 1 Experiment purpose

[0102] To investigate the anti-liver cancer effect of massoia lactone and 5-hydroxy-2-decenoic acid.

[0103] 2 Experimental methods

[0104] Experimental animal: The experimental animal used in this experiment is BALB / C (nu / nu) Nude mice, weighing 15-20 g, 4-5 weeks old, were purchased from Shanghai Bangyao Biotechnology Co., Ltd.

[0105] Cell line: Liver cancer HepG-2 cell line was purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences, using DMEM medium containing 10% fetal bovine serum, at 37°C, with a volume fraction of 5% CO 2 , Routine culture under fully saturated humidity conditions, and the cells in the logarithmic growth phase were taken for experiments.

[0106] Massoia lactone is isolated from fermented Cordyceps mycelium powder in the laboratory. 5-Hydroxy-2-decenoic acid is obtained by hydrolysis of massoia lactone base and prepared by column chromatography. The structure is obtained by NMR and MS, the purity ...

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Abstract

The invention relates to novel application of massoia lactone and 5-hydroxy-2-caproleic acid. An animal model test proves that for a CCL4 induced mouse hepatic fibrosis and liver cirrhosis model, after massoia lactone or 5-hydroxy-2-caproleic acid is used for intervention, the abnormal liver function can be remarkably improved, the serum aspartate amino transferase, glutamic-pyruvic transaminase and the total bilirubin level are lowered, the content of hepatic tissue hydroxyproline is decreased, hepatic stellate cell activation is inhibited, the type I collagen expression is reduced, and liver tissue collagen deposition is restrained; for a HepG-2 nude-mouse transplanted tumor model, after massoia lactone or 5-hydroxy-2-caproleic acid is used for intervention, increase of the weight and size of transplantation tumors can be remarkably inhibited. Accordingly, the two compounds can be used for preparing liver fibrosis, liver cirrhosis and liver cancer resistance drugs.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of massoia lactone and 5-hydroxy-2-decenoic acid in the preparation of drugs for resisting liver fibrosis, chronic liver disease, liver cirrhosis and liver cancer. Background technique [0002] The Chinese name of Massoia lactone is 5,6-dihydro-6-pentyl-2H-pyran-2-one (see the chemical structure figure 1 ), present in Masoya osmanthus oil, fermented Cordyceps mycelia, liquor, wine, sweet-scented osmanthus and sugar cane molasses, and has antibacterial, anti-infective, and immune-regulating effects. In addition, because of its creamy, coconut, spicy, and celery aroma, it is also often used as a precious spice for butter, cream, vanilla, peach, almond, fruit, nut, and tobacco flavor formulations. 5-Hydroxy-2-decenoic acid is the product after the hydrolysis of the lactone ring of massoia lactone (chemical structure see figure 1 ), which are only reported in the s...

Claims

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Application Information

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IPC IPC(8): A61K31/366A61K31/201A61P1/16A61P35/00
CPCA61K31/201A61P1/16A61P35/00
Inventor 刘平刘伟徐莹戚胜兰陈佳美肖准胡永红
Owner SHUGUANG HOSPITAL AFFILIATED WITH SHANGHAI UNIV OF T C M
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