Novel application of massoia lactone and 5-hydroxy-2-caproleic acid
A technology of massoia lactone and decenoic acid, which is applied in the field of medicine, can solve the problems of few reports on pharmacological and pharmacological effects, and achieve the effect of reducing liver collagen fiber deposition, reducing light proline content, and good therapeutic effect
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Embodiment 1
[0039] 1 Experiment purpose
[0040] Study on the anti-fibrosis effect of massoia lactone and 5-hydroxy-2-decenoic acid
[0041] 2 Experimental methods
[0042] Experimental animals: The experimental animals used in the present invention are C57BL / 6 mice, weighing 20-22 g, male, provided by the Experimental Animal Center of Shanghai University of Traditional Chinese Medicine.
[0043]Carbon tetrachloride (CCL4) and olive oil used in the experiment were purchased from Sinopharm Chemical Reagent Co., Ltd.; massoia lactone was isolated from fermented Cordyceps mycelium powder in the laboratory, and 5-hydroxy-2-decenoic acid was The massoia lactone is hydrolyzed and converted by base and prepared by column chromatography, the structure is determined by NMR and MS, and the purity is greater than 98% by HPLC area normalization method.
[0044] Forty C57BL mice, SPF grade, were divided into normal group (Control, n=10), liver fibrosis model group (CCL4, n=10), massoia lactone group...
Embodiment 2
[0070] 1 Experiment purpose
[0071] Investigation of the Anticirrhosis Effects of Massoia Lactone and 5-Hydroxy-2-Decenoic Acid
[0072] 2 Experimental methods
[0073] Experimental animals: The experimental animals used in the present invention are C57BL / 6 mice, weighing 20-22 g, male, provided by the Experimental Animal Center of Shanghai University of Traditional Chinese Medicine.
[0074] Carbon tetrachloride (CCL4) and olive oil used in the experiment were purchased from Sinopharm Chemical Reagent Co., Ltd.; massoia lactone was isolated from fermented Cordyceps mycelium powder in the laboratory, and 5-hydroxy-2-decenoic acid was The massoia lactone is hydrolyzed and converted by base and prepared by column chromatography, the structure is determined by NMR and MS, and the purity is greater than 98% by HPLC area normalization method.
[0075] Forty C57BL mice, SPF grade, were divided into normal group (Control, n=10), liver cirrhosis model group (CCL4, n=10), massoia la...
Embodiment 3
[0101] 1 Experiment purpose
[0102] To investigate the anti-liver cancer effect of massoia lactone and 5-hydroxy-2-decenoic acid.
[0103] 2 Experimental methods
[0104] Experimental animal: The experimental animal used in this experiment is BALB / C (nu / nu) Nude mice, weighing 15-20 g, 4-5 weeks old, were purchased from Shanghai Bangyao Biotechnology Co., Ltd.
[0105] Cell line: Liver cancer HepG-2 cell line was purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences, using DMEM medium containing 10% fetal bovine serum, at 37°C, with a volume fraction of 5% CO 2 , Routine culture under fully saturated humidity conditions, and the cells in the logarithmic growth phase were taken for experiments.
[0106] Massoia lactone is isolated from fermented Cordyceps mycelium powder in the laboratory. 5-Hydroxy-2-decenoic acid is obtained by hydrolysis of massoia lactone base and prepared by column chromatography. The structure is obtained by NMR and MS, the purity ...
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