Method for measuring content of free hemoglobin in blood bag through using multi-position fluorescence spectra

A fluorescence spectrum and hemoglobin technology, which is applied in the field of stoichiometry for concentration analysis of complex spectral solutions, can solve problems such as spectral nonlinearity, and achieve the effect of strong measurement pertinence, solving non-destructive testing problems, and suppressing nonlinear effects.

Inactive Publication Date: 2017-11-17
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, it is affected by the scattering of blood, which will lead to nonlinearity of the spectrum. To solve this problem, this method proposes a method for measuring free hemoglobin content in blood bags by multi-position fluorescence spectroscopy.

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  • Method for measuring content of free hemoglobin in blood bag through using multi-position fluorescence spectra
  • Method for measuring content of free hemoglobin in blood bag through using multi-position fluorescence spectra
  • Method for measuring content of free hemoglobin in blood bag through using multi-position fluorescence spectra

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Embodiment 1

[0050] In the method for measuring free hemoglobin content in blood bags by multi-position fluorescence spectroscopy provided by the embodiments of the present invention, the devices used are such as figure 1 As shown, it includes: a fluorescent excitation light source 1 , a blood bag 3 , a displacement platform 4 and a spectrum receiving device 5 .

[0051] Wherein, ensure that the light outlet of the fluorescence excitation light source 1 and the incident slit of the spectrum receiving device 5 are close to the blood bag 3, and the fluorescence excitation light source 1 excites the blood sample in the blood bag 3 at the first position a, and the blood sample in the blood bag 3 is excited by the spectrum receiving device 5. The device 5 collects fluorescence spectra. Then the fluorescence excitation light source 1 is controlled to move to the second position b by the displacement platform 4, and the fluorescence spectrum at this position is collected, and the fluorescence exc...

Embodiment 2

[0060] The difference between the embodiment of the present invention and embodiment 1 is only that the fluorescence excitation light source 1 and the movement mode of the spectrum receiving device 5 are different, see the following description for details:

[0061] see figure 2 , ensure that the light outlet of the fluorescence excitation light source 1 and the incident slit of the spectrum receiving device 5 are close to the blood bag 3, the fluorescence excitation light source 1 excites the blood sample in the blood bag 3, and the spectrum receiving device 5 is in the first position a Collect fluorescence spectra. Then the spectrum receiving device 5 is controlled by the displacement platform 4 to move to the second position b, and the fluorescence spectrum at the second position b is collected; the spectrum receiving device 5 is controlled by the displacement platform 4 to move to the nth position, and the fluorescence at the nth position is collected spectrum.

[0062]...

Embodiment 3

[0066] During specific implementation, due to the limitation of the space structure, the situation that the fluorescence excitation light source 1 and the spectrum receiving device 5 cannot be close to the blood bag 3 may occur. , as the incident fiber 2 and the exit fiber 6 .

[0067] see image 3 , the fluorescence excitation light source 1 excites the blood sample in the blood bag 3 through the incident optical fiber 2, the fluorescence spectrum is collected by the spectrum receiving device 5 through the outgoing optical fiber 6, the incident optical fiber 2 and the outgoing optical fiber 6 are respectively close to the blood bag 3, and the position a is The first position of the incident optical fiber 2, the spectrum receiving device 5 collects the fluorescence spectrum at this position; then the incident fiber 2 is controlled by the displacement platform 4 to move to the second position b, and the spectrum receiving device 5 collects the fluorescence spectrum at this posi...

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Abstract

The invention discloses a method for measuring the content of free hemoglobin in a blood bag through using multi-position fluorescence spectra. The method comprises the following steps: the light exit port of a fluorescence excitation light source and the entrance slit of a spectrum receiving device are close to the blood bag, the fluorescence excitation light source excites a blood sample to generate fluorescence, a displacement platform controls the fluorescence excitation light source to move to multiple positions, and the light spectrum receiving device receives fluorescence spectra; the fluorescence spectra in the multiple positions are normalized, and chemical examination data is combined to establish a mathematical model; and the fluorescence spectra of an unknown blood sample are acquired in the multiple positions and are normalized, and the obtained normalization result is substituted into the mathematical model to calculate the content of the free hemoglobin. The method has the advantages of fullness in use of the high pertinence of fluorescence, increase of the information content of the free hemoglobin due to multi-position fluorescence spectra, great inhibition of the influences brought by the scattering of the blood, and improvement of the content analysis precision of the blood.

Description

technical field [0001] The invention relates to the field of concentration analysis stoichiometry of complex spectral solutions, in particular to a method for measuring free hemoglobin content in blood bags by multi-position fluorescence spectroscopy. Background technique [0002] In the existing technology, the more mature technology is to detect the content of free hemoglobin in the blood bag through chemical testing, which has the outstanding advantage of high accuracy, but the chemical testing method needs to open the blood bag to take out the sample for testing, which cannot meet the needs of fast, Non-contact and pollution-free requirements. [0003] Through the study of fluorescence spectrum, it is found that it is also possible to detect the content of free hemoglobin in blood bags due to its non-contact, non-polluting, and highly targeted characteristics. [0004] However, it is affected by the scattering of blood, which will lead to nonlinearity of the spectrum. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 林凌甄建芸王玉宇王艳军张盛昭罗永顺李刚
Owner TIANJIN UNIV
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