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Novel subarachnoid hemorrhage model manufacturing method

A technology of subarachnoid cavity and manufacturing method, which is applied in medical science, veterinary instruments, veterinary surgery, etc., can solve the problems of insufficient attention to the accuracy of SAH models, and achieve the goals of shortening modeling time, promoting efficiency, and improving efficiency Effect

Inactive Publication Date: 2017-11-21
彭建华
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  • Abstract
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Problems solved by technology

At present, there is no uniform standard for the selection of SAH animal models, and the accuracy of SAH models is slightly insufficient.

Method used

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  • Novel subarachnoid hemorrhage model manufacturing method
  • Novel subarachnoid hemorrhage model manufacturing method
  • Novel subarachnoid hemorrhage model manufacturing method

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Embodiment Construction

[0025] The present invention will be further described below in conjunction with the accompanying drawings.

[0026] A method for making a novel subarachnoid hemorrhage model, comprising the following steps:

[0027] (1) Selection of biological model: Wild-type adult healthy male C57BL / 6J mice, aged 8-10 weeks, were fasted overnight before surgery;

[0028] (2) Biological model preparation: intraperitoneal injection of pentobarbital sodium for anesthesia, the injection volume is selected according to 50 mg / kg, placed on the animal operating table in a supine position, and the body temperature is maintained at about 37°C, the right lower abdomen is exposed, and the skin is prepared Disinfect the skin, bluntly expose the right femoral artery, insert a PE-10 tube into the right femoral artery under a microscope, connect it to the animal function experiment system to detect the mean arterial pressure, suture the skin, and adjust the body position;

[0029] (3) Put in the detectio...

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Abstract

The invention relates to a method for making a novel subarachnoid hemorrhage model, and belongs to the technical field of biological experiment supplies. The technical solution of the present invention is: a method for making a novel subarachnoid hemorrhage model, comprising the following steps: selection of biological models, preparation of biological models, insertion of detection probes, model making, postoperative care of biological models, and optimization of biological models. The manufacturing method of model technology has the advantages of shortening the modeling time, accurate modeling simulation and improving the efficiency of modeling.

Description

technical field [0001] The invention relates to a method for making a novel subarachnoid hemorrhage model, belonging to the technical field of biological experiment supplies. Background technique [0002] Spontaneous subarachnoid hemorrhage (Subarachnoid hemorrhage, SAH) is a syndrome caused by sudden rupture of cerebral blood vessels caused by certain diseases and blood flow to the subarachnoid space. Cracking is the main reason. With the improvement of early diagnosis and treatment, the risk of aneurysm rebleeding and the incidence of cerebral vasospasm have been significantly reduced. However, the early mortality rate of SAH remains high. Early brain injury (Early brain injury, EBI) refers to a series of pathophysiological changes caused by increased intracranial pressure, cerebral edema, and neuroinflammation within 72 hours after SAH. Unlike the reversal of cerebral vasospasm that does not improve the prognosis, the view that EBI affects the prognosis of SAH patients...

Claims

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Application Information

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IPC IPC(8): A61D1/00A61D7/00
CPCA61D1/00A61D7/00
Inventor 彭建华
Owner 彭建华
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