A method for constructing an enzyme-responsive multifunctional nanocoating
A nano-coating and construction method technology, applied in coatings, medical formulations, surgery, etc., can solve the problems of surface modification of nano-coating cardiovascular materials, difficulty in maintaining long-term function of biological coatings, loss of biological activity, etc. , to achieve the effect of simple construction process and fixing method, wide application range, and functional modification
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Embodiment 1
[0033] see figure 1 , the first specific embodiment of the present invention is a method for constructing an enzyme-responsive nano-coating, the steps of which are:
[0034] A) After the surface of 316L stainless steel was polished and cleaned, it was immersed in a dopamine solution with a concentration of 2 mg / ml to react for 12 hours. After ultrasonic cleaning with double distilled water, the above steps were repeated twice to obtain three layers of polydopamine coating surface, dry at 37°C;
[0035] B) Soak the sample deposited with polydopamine coating in step A) in 0.1 mg / ml avidin solution, let it stand for reaction at 37°C for 8 hours, then wash the sample with double distilled water, and save it for later use;
[0036] C) The biotinylated heparin sodium solution with a concentration of 10 mg / ml and the polyethyleneimine solution with a concentration of 1 mg / ml were blended in equal volumes, and ultrasonically treated for 5 minutes at room temperature to obtain a nanopar...
Embodiment 2
[0044] A method for constructing an enzyme-responsive nano-coating, the steps of which are:
[0045] A) After the surface of 316L stainless steel was polished and cleaned, it was immersed in a dopamine solution with a concentration of 2 mg / ml to react for 12 hours. After ultrasonic cleaning with double distilled water, the above steps were repeated twice to obtain three layers of polydopamine coating surface, dry at 37°C;
[0046] B) Soak the sample deposited with polydopamine coating in step A) in 0.5 mg / ml avidin solution, let it stand for reaction at 37°C for 8-24 hours, then wash the sample with double distilled water, and store it until use;
[0047] C) The biotinylated heparin sodium solution with a concentration of 20 mg / ml and the polyethyleneimine solution with a concentration of 2 mg / ml were blended in equal volumes, and ultrasonically treated for 5 minutes at room temperature to obtain a nanoparticle suspension. Submerge the sample obtained in step B into the nano...
Embodiment 3
[0055] A method for constructing an enzyme-responsive nano-coating, the steps of which are:
[0056] A) After the surface of 316L stainless steel was polished and cleaned, it was immersed in a dopamine solution with a concentration of 2 mg / ml to react for 12 hours. After ultrasonic cleaning with double distilled water, the above steps were repeated twice to obtain three layers of polydopamine coating surface, dry at 37°C;
[0057] B) Soak the sample deposited with polydopamine coating in step A) in 0.3 mg / ml avidin solution, let it stand for reaction at 37°C for 12 hours, then wash the sample with double distilled water, and save it for later use;
[0058] C) The biotinylated heparin sodium solution with a concentration of 15 mg / ml and the polyethyleneimine solution with a concentration of 1.5 mg / ml were blended in equal volumes, and ultrasonically treated for 5 minutes at room temperature to obtain a nanoparticle suspension. Submerge the sample obtained in step B into the na...
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