Application of engelhardia roxburghiana extract in preparing farnesol X receptor agonist

A technology of extract and farnesol, which is applied in the direction of drug combination, plant raw materials, medical preparations containing active ingredients, etc., can solve the problems that the application of Huang Qi extract has not been reported.

Active Publication Date: 2017-11-28
CATCH BIO SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the application of Huangqi extract in the preparation of medicines for the treatment of cholestatic liver disease has not been reported.

Method used

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  • Application of engelhardia roxburghiana extract in preparing farnesol X receptor agonist
  • Application of engelhardia roxburghiana extract in preparing farnesol X receptor agonist

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 Preparation of Huangqi Extract ER

[0050] Take the dried leaves of Huangqi, extract with 55% ethanol aqueous solution as solvent, the ratio of solid to liquid is 1:10, reflux extraction 3 times, extract for 1 hour each time, combine the extracts, concentrate under reduced pressure, and dry to obtain Huangqi extract ER.

Embodiment 2

[0051] Example 2 Preparation of ER-A1, ER-A2, ER-A3, ER-A4 from Huangqi Extract

[0052] The Huangqi extract ER prepared in Example 1 was dissolved in hot water, and it was prepared into an aqueous solution with a concentration of 20 mg / mL, with a sample loading volume of 300 mL, purified by LX-2000 macroporous resin column chromatography, and the diameter of the macroporous resin column was 8cm, the column volume is 3.5L, water is used as mobile phase A, ethanol is used as mobile phase B, the flow rate of gradient elution is 3BV / h, and the gradient elution is carried out according to the following procedure: first use A:B volume ratio of 100% : 0% mobile phase to elute 3BV, collect the eluate, concentrate and dry to obtain the Huangqi extract ER-A1, then use A:B volume ratio of 80%: 20% mobile phase to elute 4BV, collect and elute solution, concentrated under reduced pressure, and dried to obtain ER-A2, the extract of Huang Qi, and then eluted 4BV with a mobile phase with a...

Embodiment 3

[0053] Example 3 Preparation of ER-B1 and ER-B2 of Huangqi Extract

[0054] Dissolve the Huangqi extract ER prepared in Example 1 with hot water, prepare an aqueous solution with a concentration of 20 mg / mL, add 2 times the volume of ethyl acetate to extract 3 times, combine the ethyl acetate layers, concentrate under reduced pressure, and dry , to obtain Huang Qi extract ER-B1; the combined water layers were concentrated under reduced pressure and dried to obtain Huang Qi extract ER-B2.

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Abstract

The invention relates to application of engelhardia roxburghiana extract in preparing a medicament for treating cholestatic liver disease. The engelhardia roxburghiana extract ER and ER-A2 have certain FXR activating action, and the engelhardia roxburghiana extract ER-A3 and ER-B2 have relatively strong activating action on FXR and can be directly used as a farnesol X receptor agonist. In addition, the engelhardia roxburghiana extract ER-A3 and ER-B1 prepared by the invention have remarkable inhibiting action on activity rise of Tbil, ALT, APL and gamma-GT caused by cholestasis induced by ANIT, obviously improve the hepatocyte damage, inflammatory cell infiltration and bile excretion caused by ANIT and have relatively good therapeutic action on cholestatic liver disease, and can be used as a potential drug for treating cholestatic liver disease.

Description

technical field [0001] The invention belongs to the field of natural medicines, and in particular relates to the application of Huangqi extract in the preparation of medicines for treating cholestatic liver diseases. Background technique [0002] Cholestatic liver disease is a group of common clinical diseases with cholestasis as the main manifestation. Causes bile excretion disorder, bile flow reduction, bile components back into the blood, causing a series of organic damage, leading to jaundice, skin itching, glucose metabolism and lipid metabolism disorders, etc., mainly involving middle-aged and elderly people, pregnant women, children etc., seriously endanger people's health, even death. [0003] Chinese patent document CN 103181952 A discloses the use of Huang Qi extract in the preparation of medicines for preventing and treating autoimmune suppression diseases. [0004] However, the application of Huangqi extract in the preparation of medicines for treating cholesta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/52A61P1/16A61K127/00
CPCA61K36/52A61K2236/333A61K2236/39A61K2236/55
Inventor 温尧林刘晓宇
Owner CATCH BIO SCI & TECH
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