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Application of Vine Tea Extract in Preparation of Farnesoid X Receptor Agonist

A technology of rattan tea extract and extract liquid, applied in the application field of medicine, can solve the problems of no report on the application of rattan tea extract and the like

Active Publication Date: 2021-03-02
CATCH BIO SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there is no report on the application of vine tea extract in the preparation of medicines for the treatment of cholestatic liver disease

Method used

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  • Application of Vine Tea Extract in Preparation of Farnesoid X Receptor Agonist
  • Application of Vine Tea Extract in Preparation of Farnesoid X Receptor Agonist

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 Preparation of vine tea extract AG

[0050] Take the dried vine tea leaves, extract with 55% ethanol aqueous solution as solvent, the ratio of solid to liquid is 1:10, reflux extraction for 3 times, extract for 1 hour each time, combine the extracts, concentrate under reduced pressure, and dry to get vine tea extract AG .

Embodiment 2

[0051] Example 2 Preparation of vine tea extracts AG-A1, AG-A2, AG-A3

[0052] The rattan tea extract AG prepared in Example 1 was dissolved in hot water, and it was prepared into an aqueous solution with a concentration of 20 mg / mL, with a sample loading volume of 300 mL, purified by LX-2000 macroporous resin column chromatography, and the diameter of the macroporous resin column was 8cm, the column volume is 3.5L, water is used as mobile phase A, ethanol is used as mobile phase B, the flow rate of gradient elution is 3BV / h, and the gradient elution is carried out according to the following procedure: first use A:B volume ratio of 100% : 0% mobile phase to elute 3BV, collect the eluate, concentrate and dry to obtain vine tea extract AG-A1, then use A:B volume ratio of 50%:50% mobile phase to elute 4BV, collect and elute solution, concentrated under reduced pressure and dried to obtain vine tea extract AG-A2, and then use A:B volume ratio of 10%:90% mobile phase to elute 4BV...

Embodiment 3

[0053] Example 3 Preparation of vine tea extracts AG-B1 and AG-B2

[0054] Dissolve the vine tea extract AG prepared in Example 1 with hot water, prepare an aqueous solution with a concentration of 20 mg / mL, add 2 times the volume of ethyl acetate to extract 3 times, combine the ethyl acetate layers, concentrate under reduced pressure, and dry , to obtain vine tea extract AG-B1; combine the aqueous layers, concentrate under reduced pressure, and dry to obtain vine tea extract AG-B2.

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PUM

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Abstract

The invention relates to the application of vine tea extract in the preparation of medicine for treating cholestatic liver disease. The vine tea extract AG prepared by the present invention has a certain FXR activation effect, and the vine tea extract AG-A2, AG-B1 and dihydromyricetin have a strong activation effect on FXR, and can be used as a potential farnesoid X receptor. body agonist; vine tea extract AG-A2, AG-B1 and dihydromyricetin prepared by the present invention have significant inhibition on the Tbil, ALT, APL and gamma-GT activity increase caused by cholestasis induced by ANIT It can significantly improve the inhibition of liver cell injury, inflammatory cell infiltration and bile excretion caused by ANIT, and has a good therapeutic effect on ANIT-induced cholestatic hepatitis, and can be used as a potential drug for the treatment of cholestatic liver disease.

Description

technical field [0001] The invention belongs to the field of natural medicines, and in particular relates to the application of rattan tea extracts in the preparation of medicines for treating cholestatic liver disease. Background technique [0002] Cholestatic liver disease is a group of common clinical diseases with cholestasis as the main manifestation. Causes bile excretion disorder, bile flow reduction, bile components back into the blood, causing a series of organic damage, leading to jaundice, skin itching, glucose metabolism and lipid metabolism disorders, etc., mainly involving middle-aged and elderly people, pregnant women, children etc., seriously endanger people's health, even death. [0003] Anti-chronic liver fibrosis effect of vine tea extract ("Chinese Journal of Experimental Formulas", 2011, 17 (3), 132-134) discloses that vine tea extract can significantly reduce the abnormally elevated serum levels of liver fibrosis rats. MDA content and GSH-Px content i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/87A61K31/352A61P1/16
CPCA61K31/352A61K36/87A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61K2236/55
Inventor 温尧林刘晓宇
Owner CATCH BIO SCI & TECH
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