Application of vine tea extract in preparing farnesol X receptor agonist

A technology of vine tea extract and receptor agonist, which is applied in the application field of medicine, and can solve the problems that the application of vine tea extract has not been reported.

Active Publication Date: 2017-11-28
CATCH BIO SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there is no report on the application of vine tea extract in the preparation of medicines for the treatment of cholestatic liver disease

Method used

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  • Application of vine tea extract in preparing farnesol X receptor agonist
  • Application of vine tea extract in preparing farnesol X receptor agonist

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 Preparation of vine tea extract AG

[0050] Take the dried vine tea leaves, extract with 55% ethanol aqueous solution as solvent, the ratio of solid to liquid is 1:10, reflux extraction for 3 times, extract for 1 hour each time, combine the extracts, concentrate under reduced pressure, and dry to get vine tea extract AG .

Embodiment 2

[0051] Example 2 Preparation of vine tea extracts AG-A1, AG-A2, AG-A3

[0052] The rattan tea extract AG prepared in Example 1 was dissolved in hot water, and it was prepared into an aqueous solution with a concentration of 20 mg / mL, with a sample loading volume of 300 mL, purified by LX-2000 macroporous resin column chromatography, and the diameter of the macroporous resin column was 8cm, the column volume is 3.5L, water is used as mobile phase A, ethanol is used as mobile phase B, the flow rate of gradient elution is 3BV / h, and the gradient elution is carried out according to the following procedure: first use A:B volume ratio of 100% : 0% mobile phase to elute 3BV, collect the eluate, concentrate and dry to obtain vine tea extract AG-A1, then use A:B volume ratio of 50%:50% mobile phase to elute 4BV, collect and elute solution, concentrated under reduced pressure and dried to obtain vine tea extract AG-A2, and then use A:B volume ratio of 10%:90% mobile phase to elute 4BV...

Embodiment 3

[0053] Example 3 Preparation of vine tea extracts AG-B1 and AG-B2

[0054] Dissolve the vine tea extract AG prepared in Example 1 with hot water, prepare an aqueous solution with a concentration of 20 mg / mL, add 2 times the volume of ethyl acetate to extract 3 times, combine the ethyl acetate layers, concentrate under reduced pressure, and dry , to obtain vine tea extract AG-B1; combine the aqueous layers, concentrate under reduced pressure, and dry to obtain vine tea extract AG-B2.

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Abstract

The invention relates to an application of vine tea extract in preparing cholestatic liver disease. The vine tea extract AG prepared by the method has a certain FXR activating action. The vine tea extract AG-A2 and AG-B1 and dihydromyricetin has relatively high activating action on FXR and can be used as a potential farnesol X receptor agonist. The vine tea extract AG-A2 and AG-B1 prepared by the invention and dihydromyricetin have an obvious inhibiting effect on activity rise of Tbil, ALT, APL and gamma-GT caused by cholestasis induced by ANIT, can obviously inhibit hepatocyte damage and inflammatory cell infiltration induced by ANIT and bile drainage, have a relatively good treatment effect on cholestasis type hepatitis induced by ANIT and can be used as a potential drug for treating cholestatic liver disease.

Description

technical field [0001] The invention belongs to the field of natural medicines, and in particular relates to the application of rattan tea extracts in the preparation of medicines for treating cholestatic liver diseases. Background technique [0002] Cholestatic liver disease is a group of common clinical diseases with cholestasis as the main manifestation. Causes bile excretion disorder, bile flow reduction, bile components back into the blood, causing a series of organic damage, leading to jaundice, skin itching, glucose metabolism and lipid metabolism disorders, etc., mainly involving middle-aged and elderly people, pregnant women, children etc., seriously endanger people's health, even death. [0003] Anti-chronic liver fibrosis effect of vine tea extract ("Chinese Journal of Experimental Formulas", 2011, 17 (3), 132-134) discloses that vine tea extract can significantly reduce the abnormally elevated serum levels of liver fibrosis rats. MDA content and GSH-Px content ...

Claims

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Application Information

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IPC IPC(8): A61K36/87A61K31/352A61P1/16
CPCA61K31/352A61K36/87A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61K2236/55
Inventor 温尧林刘晓宇
Owner CATCH BIO SCI & TECH
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