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Storage liquid of human umbilical cord mesenchymal stem cells

A technology for stem cells and human umbilical cord, applied in the field of stem cell biology, can solve the problems of decreased activity of human umbilical cord mesenchymal stem cells, inability to large-scale application, and limitation of large-scale production and long-distance supply of human umbilical cord mesenchymal stem cells, To achieve the effect of long-distance transportation and widespread clinical promotion, convenient transportation and temporary storage, and the effect of promoting survival and migration

Active Publication Date: 2017-12-01
刘劼 +9
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, human umbilical cord mesenchymal stem cells have been widely used clinically, but there are also problems that they cannot be used on a large scale due to storage conditions
Human umbilical cord mesenchymal stem cells age quickly at room temperature under the conditions of the commonly used storage solution at present, which limits the large-scale production and long-distance supply of human umbilical cord mesenchymal stem cells in clinical applications
In addition, in clinical applications, human umbilical cord mesenchymal stem cell preparations need to complete quality tests such as sterility and activity before they can be injected into the human body. These tests take a long time to obtain reliable results
However, the cryopreservation commonly used now has great damage to cells and cannot be directly injected into the human body after thawing. Therefore, during the quality inspection of stem cell preparations, a suitable storage solution is the basis for clinical application.
[0005] In the course of clinical application, human umbilical cord mesenchymal stem cells are often used as seed cells to gather, proliferate, differentiate, and secrete a variety of repair nutritional factors at the damaged site. However, the current storage solution lacks the necessary living conditions and nutrients, making people The activity of umbilical cord mesenchymal stem cells decreases during preservation, which in turn affects the actual therapeutic effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] (1) Primary cell culture

[0063] 1) Add an appropriate amount of complete medium to the fresh human umbilical cord specimens (donated from the Department of Obstetrics, Tongji Hospital Affiliated to Tongji University, and all the screening indicators of the donors are qualified) after digestion with digestive enzymes to terminate digestion;

[0064] 2) Add the complete medium to 10mL, pipette the dropper repeatedly to fully disperse the cells;

[0065] 3) Filter with a 70 μm cell sieve to remove undigested tissue block components;

[0066] 4) Centrifuge at 1500rpm for 10min;

[0067] 5) Use a dropper to carefully suck off the supernatant, and keep the viscous liquid part;

[0068] 6) Wash twice with complete medium, the method is the same as before;

[0069] 7) Resuspend the harvested cell pellet in complete medium and inoculate into T-25cm 2 Cell culture flask, add 5mL complete medium, place in 5% CO 2 cultured in a 37°C saturated humidity cell incubator;

[007...

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Abstract

The invention provides storage liquid of human umbilical cord mesenchymal stem cells, and belongs to the field of stem cell biology. The storage liquid belongs to a composition of stem cell storage liquid suitable for clinic application. Various active ingredients are used for optimizing the storage conditions, and comprise a mixed water solution containing sodium chloride, albumin, adenosine, angiotensin-II, lithium chloride, flavonoids compounds, heparin, andhuman umbilical cord mesenchymal stem cell sourced exosome, oligopeptide and polypeptide compounds. In the storage liquid, the cell activity of the human umbilical cord mesenchymal stem cells under the condition of 4 DEG C in 3 days is maintained at 90 percent or higher; in addition, the cell phenotype is stable; the cell activity can still be maintained at 90 percent or higher in 7 days under the condition of -20 DEG C; the cell phenotype is also stable. The storage liquid maintains the stable quality of the stem cells; the long-distance conveyance of the stem cells can be realized; the foundation is laid for the wide application of the clinic stem cells.

Description

technical field [0001] The invention relates to the field of stem cell biology, in particular to a human umbilical cord mesenchymal stem cell storage solution. Background technique [0002] As a widely used stem cell, human umbilical cord mesenchymal stem cells are easy to obtain, easy to separate, have low immunogenicity, and have the advantages of multi-directional differentiation potential. They can be applied to clinical disease treatment and anti-aging of the body . With the standardized application of stem cell technology in the field of biological therapy, especially its advantages in the treatment of chronic diseases such as hypertension and coronary atherosclerosis, it can improve the body's function and quality of life as a whole, which has been widely concerned and is becoming more and more popular. More and more clinical applications. [0003] The storage environment of human umbilical cord mesenchymal stem cells is closely related to the long-term activity of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0215A01N1/0221A01N1/0226
Inventor 刘劼朱益辉孙毅胡敏曾赤佳陆军薛婷陈彩琴廖方圆黄明辉
Owner 刘劼
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