The use of vitamin c in the preparation of culture medium

A technology of culture medium and vitamins, applied in the direction of culture process, tissue culture, cell culture active agent, etc., to achieve the effect of improving differentiation efficiency

Active Publication Date: 2020-12-11
GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] However, a stable and efficient induction differentiation system still needs to be further improved

Method used

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  • The use of vitamin c in the preparation of culture medium
  • The use of vitamin c in the preparation of culture medium

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Experimental program
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Embodiment Construction

[0042] Embodiments of the invention are described in detail below, examples of which are illustrated in the accompanying drawings. The embodiments described below by referring to the figures are exemplary and are intended to explain the present invention and should not be construed as limiting the present invention.

[0043] (1) Vc promotes the differentiation of human pluripotent stem cells into hematopoietic precursor cells

[0044] On the basis of the basal medium BM (DMEM / F12 (Hyclone)+1% ITS (insulin-transferrin-selenium, GIBCO)+vitamin C (Vc, GIBCO, 70ng / mL)) of the present application, the inventor tested whether it contained or not Effect of basal medium containing vitamin C on hematopoietic differentiation of human pluripotent stem cells. Such as figure 1 As shown in A, on the -1st day, the inventor digested human embryonic stem cells H1 with 2mg / mL Dispase (neutral protease) into small pieces, each small piece contained about 20-50 cells; About 150,000 cells were ...

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Abstract

The invention provides use of vitamin C for preparing a culture medium. The culture medium is used for promoting hematopoiesis differentiation of embryonic stem cells or induced pluripotent stem cells. Through an experiment, in a monolayer differentiation culture system of the human pluripotent stem cell hematopoiesis differentiation, the human pluripotent stem cells can be effectively promoted to be induced and differentiated as HPC of CD34+CD43+ by adding the vitamin C, and the vitamin C has the importance function for promoting the directional hematopoiesis differentiation of the human pluripotent stem cells (comprising the embryonic stem cells or induced pluripotent stem cells).

Description

technical field [0001] The present invention relates to the field of biology, in particular, the present invention relates to the use of vitamin C in the preparation of medium, the medium for promoting the hematopoietic differentiation of embryonic stem cells or induced pluripotent stem cells and the method for promoting the hematopoietic differentiation of embryonic stem cells or induced pluripotent stem cells. Background technique [0002] Hematopoietic stem cells refer to a type of cells that have self-renewal ability and can differentiate into various blood cells (red blood cells, white blood cells and platelets). Because of its hematopoietic reconstruction ability, hematopoietic stem cells are widely used in clinical treatment of various blood diseases, including: genetic diseases (such as: thalassemia, sickle cell anemia, aplastic anemia, Fanconi anemia, etc. ) and non-genetic diseases (such as: leukemia, etc.). [0003] Clinically, hematopoietic stem cell transplanta...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0789
CPCC12N5/0647C12N2500/38C12N2501/115C12N2501/15C12N2501/155C12N2501/16C12N2501/165C12N2506/02C12N2506/45
Inventor 潘光锦黄可张田李宇航廖宝剑薛燕婷
Owner GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI
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