System and method for automatically detecting acute toxicity of chemicals to daphnia magna

An automatic detection and acute toxicity technology, applied in the field of toxicology, to reduce the experimental workload and achieve accurate and reliable experimental results

Inactive Publication Date: 2017-12-15
HOHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, Daphnia magna has been widely used in the research on the safety evaluation of industrial wastewater and domestic sewage. However, using Daphnia magna

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  • System and method for automatically detecting acute toxicity of chemicals to daphnia magna

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Experimental program
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Embodiment 1

[0027] A system for automatically detecting the acute toxicity of drugs to Daphnia magna, the system includes a toxicity prediction model QSAR, a micro operating system, an exposure system, a drug storage system, a metering pump, and a drug determination system, wherein the exposure system includes an exposure unit and an exposure unit. In the parameter control interface, the specific molecular structure of the drug is first input into the toxicity prediction model QSAR, and the concentration range of the drug during the exposure period is further set according to the concentration recommended by the model. Exposure begins after the conditions set by the system meet the requirements. At the same time, the microscopic operating system and drug determination system monitor various biological indicators and drug exposure concentrations in the exposure system. Finally, all data are summarized and input into the toxicology acute toxicity processing software to obtain drug exposure. ...

Embodiment 2

[0028] Example 2 Detection of anti-inflammatory drug diclofenac toxicity

[0029] 1) Prediction of anti-inflammatory drug diclofenac toxicity:

[0030] Input the molecular structure of diclofenac into the QSAR model to obtain the 48h half inhibitory concentration EC50 and half lethal concentration LC50 of anti-inflammatory drugs predicted by the model, which are 160mg / L and 350mg / L respectively, and the exposure concentrations are determined to be 1000mg / L, 500mg / L, and 250mg / L, 100mg / L, 50mg / L, 20mg / L.

[0031] 2) Preparation of anti-inflammatory drug diclofenac stock solution:

[0032] Weigh 5 g of diclofenac, dissolve in 100 mL of methanol, vortex, and sonicate to obtain a stock solution with a concentration of 50 g / L.

[0033] 3) Preparation of test solution

[0034] Add the anti-inflammatory drug stock solution to Daphnia magna culture solution through a metering pump, set the required water quality parameters during the exposure period, the water temperature is 22°C,...

Embodiment 3

[0048] 1) Prediction of anti-inflammatory drug ibuprofen toxicity:

[0049] Input the molecular structure of ibuprofen into the QSAR model to obtain the 48h half inhibitory concentration EC of the anti-inflammatory drug predicted by the model 50 and the median lethal concentration LC 50 , were 55mg / L and 70mg / L respectively, and the exposure concentrations were determined to be 200mg / L, 100mg / L, 50mg / L, 20mg / L, 10mg / L, 1mg / L.

[0050] 2) Preparation of anti-inflammatory drug ibuprofen stock solution:

[0051] Weigh 5g of ibuprofen, dissolve in 100mL of methanol, vortex, and sonicate to obtain a stock solution with a concentration of 50g / L. 3) Preparation of test solution

[0052] Add the drug stock solution to Daphnia magna culture solution through a metering pump, set the required water quality parameters during the exposure period, water temperature 22°C, light-to-dark ratio 16h:8h, dissolved oxygen concentration not less than 4mg / L, and the actual exposure solution The ...

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Abstract

The invention discloses a system for automatically detecting acute toxicity of chemicals to daphnia magna. The system comprises a toxicity prediction model QSAR, a microscopic operating system, an exposure system, a chemical storage system, a metering pump and a chemical determination system, wherein the exposure system comprises an exposure unit and an exposure parameter control interface. The invention also discloses a method for automatically detecting the acute toxicity of the chemicals to daphnia magna. The automatic method is established on the basis of an existing experimental method, and experimental workload is greatly reduced. A sensor is connected with a computer, conditions required for exposure are controlled more stably and accurately, and the device is equipped with an alarm system, so that experimental results are more accurate and reliable; the chemical concentration during exposure is controlled more accurately by a chemical detection unit; the activity of various behaviors of the daphnia magna is monitored more comprehensively and accurately, and the toxicity indication endpoint is judged more directly and accurately.

Description

technical field [0001] The invention relates to the field of toxicology, in particular to a system and method for automatically detecting the acute toxicity of drugs to Daphnia magna. Background technique [0002] The large daphnia (Daphnia magna) generally lives in freshwater waters and belongs to the planktonic crustaceans. It is a standard test organism for aquatic toxicology. As an intermediate level connecting phytoplankton and high-trophic levels in the food chain, the zooplankton Daphnia magna plays an important role in the material cycle and energy transfer of freshwater aquatic ecosystems. At the same time, Daphnia magna has the characteristics of fast reproduction, sensitivity to environmental changes, and easy laboratory cultivation. [0003] At present, Daphnia magna has been widely used in the research on the safety evaluation of industrial wastewater and domestic sewage. However, using Daphnia magna as a test organism, there are almost no methods for automati...

Claims

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Application Information

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IPC IPC(8): G01N33/50
CPCG01N33/5014G01N33/5085
Inventor 陆光华杨皓涵吴东海闫振华刘建超
Owner HOHAI UNIV
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