Magnetic resonance imaging decoupling hydrogen spectrum method for identifying true and adulterated honey

Abstract

The invention provide a magnetic resonance imaging decoupling hydrogen spectrum method for identifying true and adulterated honey and relates to nuclear magnetic resonance spectroscopy. The method comprises the following steps: adding an identified honey sample into a buffer solution, centrifuging the prepared sample, removing supernatant liquid, and transferring the sample into a magnetic resonance resonance tube; importing a precompiled decoupling NMR spectrum pulse sequence into a nuclear magnetic resonance spectrometer; setting pulse sequence parameters and sampling parameters, and collecting data; after the sampling is finished, carrying out relevant data processing so as to obtain a decoupling spectrogram of all samples, and preprocessing corresponding experimental data, so as to obtain subsection integral data of the decoupling spectrogram; and importing the decoupling the subsection integral data into statistical analysis software, carrying out principal component analysis and orthogonal partial least square analysis so as to judge the sizes of inter-group and in-group differences of two groups, analyzing a compound which causes the inter-group differences, and judging an adulteration manner of the adulterated honey. The pulse sequence is homonuclear wide-band decoupling pure chemical displacement spectrum method, and a 1HNMR spectrum with relatively high resolution for eliminating coupled splitting can be obtained.

Description

technical field [0001] The invention relates to a nuclear magnetic resonance spectroscopy detection method, in particular to the use of one-dimensional homonuclear decoupling 1 H NMR Spectrum A NMR Decoupled H NMR Spectroscopy Method for Distinguishing True and False Honey. Background technique [0002] Honey is a natural food rich in fructose and glucose, a small amount of sucrose, maltose and other sugars and trace vitamins, which are brewed by bees from the flowers of flowering plants and combined with their own secretions. The nutritional and health effects are favored by consumers. The huge market has brought huge economic benefits, so many unscrupulous vendors pass off adulterating honey as honey to gain profits, and the methods of adulteration are rich and varied, such as mixing cheap sucrose, vegetable syrup or Glucose syrup is used to pass off honey, etc. There are also methods such as mixing low-priced honey (rape nectar, etc.) with high-priced honey (acacia nect...

AI Technical Summary

Problems solved by technology

At present, the ZS decoupling method is only used in simple chemical samples to demonstrate its decoupling effect, and has not been applied in the actual natural product samples with complex composition

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