A kind of Chinese cutworm attractant composition and its application
A technology of horoscope cutworm and composition, applied in the direction of application, attracting pests, biocides, etc., can solve the problems of loss of function, etc., and achieve the effect of strong autonomy, cost reduction, and strong trapping activity
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Embodiment 1
[0025] The Y-type olfactometer of embodiment 1 single-component synergist is measured
[0026] Preparation of a single dose of plant-derived volatiles: each compound was prepared in three concentrations of 0.1, 1, and 10 μg / μl using n-hexane as a solvent, and n-hexane was used as a control.
[0027] The sensitivities of cutworms to various reagents were measured by "Y" type olfactometer. In a room with clean air at 25°C, a relative humidity of about 70%, and a 3cm*20cm "Y" type olfactometer for biological determination. Take 10 μl of the single-component compound of the synergist and drop it on a filter paper strip, place it in one side arm of a Y-shaped olfactometer, and put an equal amount of n-hexane in the other side arm as a control. After each group was repeated, the positions of the test group and the control group were reversed to avoid experimental errors. After each test, a new filter paper strip was replaced, each reagent was repeated 3 times, and a clean "Y" tube...
Embodiment 2
[0035] Embodiment 2 Synergistic effect of different formula synergists on sex pheromone of cutworm
[0036] 1-hexanol, phenylacetaldehyde, cis-3-hexen-1-ol, heptanal, linalool and cutworm sex pheromone were prepared into 500 μg / μL n-hexane solutions. Among them, the sex pheromone of the cutworm is 9 parts of cis-7-tetradecene acetate, 0.8 part of cis-5-tetradecene acetate and 0.2 part of trans-5-tetradecene acetate. Mix the proceeds.
[0037] Use a 200 μL pipette gun to draw a certain amount of each synergist component solution and the cutworm sex pheromone solution and mix evenly to obtain the cutworm plant-derived pheromone mixture. The composition of the attractant composition is shown in Table 1.
[0038] Use a 200 μL pipette gun to draw 200 μL of the cutworm attractant composition or the sex pheromone of the cutworm and drop it on the septum of the rubber stopper cleaned with n-hexane. After the n-hexane volatilizes, drop 1 ml of dichloromethane in portions Add, after ...
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